Abstract:The value of CODEHOP RT-PCR in identification of Hantavirus gene type and molecular homology was analyzed in this study. By using CODEHOP RT-PCR to amplify L gene segment Hantavirus in the sample, two rat lung samples stored in our lab were selected. One labeled DX0901 have been tested positive for Hantaan virus, while the other labeled DX1101 have been tested positive for Seoul virus. Blast analysis and phylogenetic tree were performed after RT-PCR products of nucleocapsid genes were sequenced. The results showed that both of the amplified fragments of the two samples were 478 bp in size. The Hantaan virus DX0901 had a high homology to Hantaan virus strain Nc167, with a genetic distance of 0.142. The Seoul virus DX1101 had a high homology to Seoul virus strain ZT10, ZT71 and ZT37, and the genetic distance was 0.049. In conclusion, the method of CODEHOP RT-PCR could be effective applied for genetic determination and phylogenetic analyses of Hantavirus.
胡群,马思杰,童淑梅. CODEHOP RT-PCR方法在汉坦病毒基因型别鉴定和分子溯源中的应用[J]. 中国人兽共患病学报, 2013, 29(9): 854-857.
HU Qun,MA Si-jie,TONG Shu-mei. Application of CODEHOP RT-PCR in identification of Hantavirus gene type and molecular homology. Chinese Journal of Zoonoses, 2013, 29(9): 854-857.
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