Abstract:The aim is to establish a suitable proliferative cell culture system for TTV (Transfusion transmitted virus), and observe the ultramicro-morphology of this virus. The gastric adenocarcinoma cell SGC-7901 and gastric mucosal cell GES-1 were prepared, and the TTV in passage SGC-7901 and GES-1 were subcultured until the cytopathic effect (CPE) appeared. Dynamic detection of TCID50 and observation on transmission electron microscopy (TEM) of infected cells were carried out. After 3 generation blind passage in SGC-7901, TTV could produce obvious CPE 72 hours p.i. The TCID50 titer of TTV was getting higher from F1 101.2 to F4 106.0, and then stabilized between 106.0 and 106.8. But in GES-1, after 8 generation blind passage the CPE was still atypism. The observation of TEM showed that TTV were distributed in the cytoplasm and nucleus of the infected cells. The virions were globular and unenveloped, and the average diameter was 20 nm. SGC-7901 cells can be used as a proliferation system for TTV, and the SGC-7901 cell adapted TTV virus have been obtained in vitro.
张娜,刘学芳,李建国,张振强. TTV病毒体外培养体系的建立及超微形态观察[J]. 中国人兽共患病学报, 2013, 29(9): 858-861.
ZHANG Na,LIU Xue-fang,LI Jian-guo,ZHANG Zhen-qiang. Establishment of proliferative system and the ultramicro-morphology observation of TTV. Chinese Journal of Zoonoses, 2013, 29(9): 858-861.
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