Effects of Staphylococcus aureus isdA gene on immunity index in the epithelial cells of the host
CONG Li-xin1,LI Lei2,YU Lu2
1.Jilin Agricultural Science and Technology College, Jilin 132101, China; 2.Key Laboratory of Zoonosis Research of the Ministry of Education,Institute of Zoonosis, Jilin University, Changchun 130062, China
Abstract:In view of the important role of IsdA in colonization and invasion of S. aureus, the immunological study about co-incubation between S. aureus IsdA+/- and human epithelial cells were progressed. Epithelial cells HaCaT were affected respectively by the wild strains Newman and IsdA deletion mutants NewmanΔIsdA of S. aureus. The secretion situation of pro-inflammatory cytokine and chemokine were tested by real-time PCR, ELISA and Western Blot. Results of PCR showed that the expression of pro-inflammatory cytokines (IL-8, TNF-α and IL-6), chemokine MCP-1, the surface receptors TLR2 and TLR4, hemagglutinin C plant-1 (dectin-1), and the antibacterial peptide LL-37 were raised by S. aureus IsdA+/-; the cytokines amount of epithelial cells treated with IsdA-/- was lower than that of IsdA+/+, but epithelial cytokines levels of IsdA-/- group was higher than that of the control group; the results were consistent with that of the PCR through the detection method of ELISA and Western Blot. It's showed that S. aureus IsdA-/- could reduce the cytokines secreted by epithelial cells compared with the wild strains IsdA+/+, and the level of cell surface receptor protein transcription decreased accordingly, which hint that invasion force of the deletion mutant decline.
丛立新,李蕾,于录. 金黄色葡萄球菌isdA基因对宿主上皮细胞免疫指标的影响[J]. 中国人兽共患病学报, 2014, 30(4): 377-382.
CONG Li-xin,LI Lei,YU Lu. Effects of Staphylococcus aureus isdA gene on immunity index in the epithelial cells of the host. Chinese Journal of Zoonoses, 2014, 30(4): 377-382.
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