Age impact in mouse model of secondary hepatic alveolar echinococcus
XIA Hai-yang1, ZHAO Jie-feng1, WANG Qi-kun1, LI Li1, TANG Jing-xia2, ZHANG Shi-jie1, PENG Xin-yu1, YANG Hong-qiang1
1.Hepatobiliary Surgery, the First Affiliated Hospital, Medical College, Shihezi University, Shihezi 832008, China; 2.Department of Preventive Medicine, Medical College, Shihezi University, Shihezi 832002, China
Abstract:Age impact in mouse model of secondary hepatic alveolar echinococcus was investigated in this research. Twenty-nine 8-week-old, twenty-five 18-week-old and twenty-five 28-week-old female mice were anesthetized with 20% urethane by intraperitoneal injection and then transhepatically injected by Echinococcus multilocularis (E. m) tissue suspension through skin incision and abdominal muscle to liver in all three groups to establish mouse model of secondary hepatic alveolar echinococcus. Results showed that the survival rates for the three groups of mice were 62.1%, 84% and 68%, respectively (P>0.05). The E. m infection rates in liver were 72.2%, 71.4% and 76.5%, respectively (P>0.05). The diameter of E. m cysts in liver were 0.915±0.103 cm, 1.247±0.112 cm and 1.215±0.197 cm, respectively (P>0.05). The mass of E. m cysts in liver were 0.332±0.035 g, 0.532±0.155 g and 0.382±0.085 g, respectively (P>0.05). HE stain showed no difference in pathology. Results indicated that the establishment of secondary hepatic alveolar echinococcus model by using transhepatic injection through skin incision and abdominal muscle of 18-week-old mice was capable of simplifying operation and improving the survival rate of the mice.
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