Abstract：In this study,we analyzed the genetic characterizations and propagation paths of measles viruses prevalent in Ningbo,China from 2004 to 2013. Measles viruses were isolated from throat swab specimens from 2004 to 2013,and 450 bp fragments of C terminus of nucleoprotein (N) gene were amplified by RT-PCR. Sequence analysis was conducted of all 31 virus strains,and then compared with other measles virus strains published in GenBank. Results showed that all the 31 measles virus isolates belonged to genotype H1,6 strains (one strains in 2004 and 5 ones in 2005) belonged to H1b subtype,and others belonged to H1a subtype. Compared the 31 strains to the China 93-4 and China 94-7,the homology of 450 bp fragments of C terminus of nucleoprotein (N) gene were 97.1%-100% and 96.7%-100%,respectively. The homology of 450 bp fragments of nucleotide and nucleotide (amino acids) compared with S191 were 81.9%-92.4% and 87.2%-90.6%,respectively. It’s suggested that genotype H1 measles virus circulated in Ningbo from 2004 to 2013,and H1a was the predominant epidemic strain,and H1b strain was existed as well. There are five different transmission chains of H1a subtype caused measles co-circulations in Ningbo.
[1]Fu Y,Ni HX,Liu JY,et al. Genetic characterization of H and N gene from wild-type measles viruses isolated in Ningbo in 2008[J]. Chin J Health Lab Technol,2011,19(8): 1754-1756. (in Chinese) 傅燕,倪红霞,胡逢蛟,等. 宁波2008年麻疹病毒分离株H和N基因特征分析[J]. 中国卫生检验杂志,2011,19(8):1754-1756. [2]Rota PA,Bellini WJ. Update on the global distribution of genotypes of wild type measles viruses[J]. J Infect Dis,2003,187(1): 270-276. DOI: 10. 1086/368042 [3]Magurano F,Fortuna C,Baggieri M,et al. Molecular epidemiology of measles virus in Italy during 2008[J]. Ann Ist Super Sanita,49(1): 50-55. DOI: 10. 4415/A-NN13-01-09 [4]Liu L,Sun L,He JL,et al. Sequence analysis on N gene of measles virus isolated in Sichuan Province,2011[J]. J Prevent Med Inform,2012,28(6): 426-429. (in Chinese) 刘李,孙莉,何吉兰,等. 2011 年上半年四川省麻疹病毒分离株N 基因序列分析[J]. 预防医学情报杂志,2012,28( 6): 426-429. [5]Wang CY,Xu ST,Xiong P,et al. Analysis of epidemiological characteristics of measles virus isolated in Shandong Province from 2005 to 2008[J]. Chin J Vaccine Immun,2011,17(1): 22-25. (in Chinese) 王常银,许松涛,熊萍,等. 山东省2005-2008 年麻疹病毒流行特征分析[J]. 中国疫苗和免疫,2011,17(1): 22-25. [6]Fu Y,Ni HX,Liu JY,et al. Genetic characteristic of wild-type measles viruses isolated in Ningbo from 2004 to 2008[J]. Chin J Vaccine Immun,2009,15(4): 319-322. (in Chinese) 傅燕,倪红霞,刘健毅,等. 宁波2004-2008年麻疹野病毒分离株基因特征分析[J]. 中国疫苗和免疫,2009,15(4): 319-322. [7]Yan JY,Lu YY,Zhang JH,et al. Genetic and immunogenic characteristics of measles virus isolated in Zhejiang Province[J]. Virologica Sin,2006,21(1): 1-5. (in Chinese) 严菊英,卢亦愚,张建华,等. 浙江省麻疹病毒分离株的基因特性与免疫原性研究[J]. 中国病毒学,2006,21(1): 1-5. [8]Li P,Si Y,Guan RH,et al. Study on pathogenic strain and vaccination-related factors of measles circulating in Shanxi province[J]. Chin J Public Health,2007,23(9): 1122-1124. (in Chinese) 李平,司源,关蓉晖,等. 陕西省麻疹病毒基因分型及疫苗免疫因素分析[J]. 中国公共卫生,2007,23(9): 1122-1124. [9]Xu WQ,Yang ZD,Chen L,et al. Isolation of wild-type measles virus and immuno-effect analysis of current measles vaccine[J]. Chin J Vaccine Immun,2008,14(3): 198-202. (in Chinese) 徐闻青,杨忠东,陈蕾,等. 麻疹病毒分离及现行疫苗免疫效果分析[J]. 中国疫苗和免疫,2008,14(3): 198-202. [10]Buckland R,Wild TF. Is CD46 the cellular receptor for measles virus?[J]. Virus Res,1997,48(1): 1-9. DOI: 10. 1016/S0168-1702(96)01421-9
2014, Vol. 30 
