Characterization and prevalence of Klebsiella pneumonia carrying the carbapenemase gene in a hospital in Shanxi Province, China
PANG Hui1, 2, 3, GONG Yan-e4, YUAN Min2, 5, JIANG Yi2, 5, GUO Bu-ping4, GONG Lin2, LI Shui-xian1, LI Juan2, 5, WAN Kang-lin2, 5
1. Department of Immunology, Changzhi Medical College, Changzhi 046000, China; 2. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 102206, China; 3. Department of Immunology, Xiangya School of Medicine, Central South University, Changsha 410078, China; 4. Department of Laboratory Medicine, Changzhi Heping Hospital Affiliated to Changzhi Medical College, Changzhi 046000, China; 5. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310000, China
Abstract:Klebsiella pneumonia (K. pneumonia) is one of the most common nosocomial pathogens and can cause severe infections in diverse organs and tissues of human body. To assess the characterization and prevalence of K. pneumonia carrying carbapenemase genes, K. pneumonia strains were isolated from a hospital in Changzhi, Shanxi Province, China, between 2008 and 2012. The drug susceptibility test (DST) of the isolates was determined by the agar disc diffusion and E-test. Carba NP test was performed to confirm the production of carbapenemases. The known carbapenemase genes were screened by PCR and DNA sequencing. Carbapenem resistant strains were analyzed by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). A total of 9 (0.69%) resistant strains from 1 297 clinical K. pneumonia isolates engendered group A carbapenemase and demonstrated K. pneumonia carbapenemase gene positive, while carbapenemase genes were all negative among the remaining isolates. MLST confirmed that all of the 9 strains belonged to ST11 genotype. Moreover, by means of PFGE patterns analysis, it could be concluded that there might be two transmissions by the resistant K. pneumonia carrying carbapenemase gene in the hospital, in which one was in 2010 and the other was in 2012. Intensive surveillance and strict infection control policy should be carried out in this area of China.
庞慧, 弓艳娥, 袁敏, 蒋毅, 郭步平, 龚林, 李水仙, 李娟, 万康林. 山西省某医院发现的携带碳青霉烯酶基因的肺炎克雷伯菌的流行特征研究[J]. 中国人兽共患病学报, 2014, 30(11): 1110-1115.
PANG Hui, GONG Yan-e, YUAN Min, JIANG Yi, GUO Bu-ping, GONG Lin, LI Shui-xian, LI Juan, WAN Kang-lin. Characterization and prevalence of Klebsiella pneumonia carrying the carbapenemase gene in a hospital in Shanxi Province, China. Chinese Journal of Zoonoses, 2014, 30(11): 1110-1115.
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