Nested-PCR and LAMP in detection of B. burgdorferi in ticks
ZHANG Lin,HOU Xue-xia,GENG Zhen,HAO Qin
State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Abstract：Nested-PCR and loop-mediated isothermal amplification (LAMP) were applied to identify the Borrelia burgdorferi (B. burgdorferi) in ticks in this study. A total of 112 adult ixodes were collected from vegetation in a forest area and farm animals in Xunhua County, Qinghai Province and Xinbin County, Liaoning Province. The ticks were examined for the presence of B. burgdorferi by nested-PCR and LAMP. Results showed that 12 in 51 samples were found positive in Xunhua County (23.53%). While positive rate in Xinbin County was 29.51% with 18 samples positive in 61 samples. In total of 112 tick samples, the PCR-positive rate was 17.86% with 20 positive samples identified, whereas 15 positive samples were confirmed with positive rate of 13.39 % by LAMP assay. There was no significant difference between the two assays (Х2=0.85, P＞0.05). Results suggest that both nested-PCR and LAMP could be used in identifying B. burgdorferi in ticks. Combination of these two assays could improve the testing results. This is the first report of B. burgdorferi in ticks in Xunhua and Xinbin counties, and helps to complete the database of the infection rate of B. burgdorferi in ticks in the widely-forested area of China.
张琳,侯学霞,耿震,郝琴. 巢式PCR与LAMP方法在蜱的莱姆病螺旋体检测中的应用[J]. 中国人兽共患病学报, 2014, 30(12): 1192-1195.
ZHANG Lin,HOU Xue-xia,GENG Zhen,HAO Qin. Nested-PCR and LAMP in detection of B. burgdorferi in ticks. Chinese Journal of Zoonoses, 2014, 30(12): 1192-1195.
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2014, Vol. 30 
