免疫人群乙肝抗体阳性率调查及乙肝核心抗体检测方法探讨

doi:10.3969/cjz.j.issn.1002-2694.2015.03.022

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (439 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的调查我院检测人群乙型肝炎病毒表面抗体(HBsAb)及乙型肝炎病毒核心抗体(HBcAb)阳性率,并探讨检测方法选择及检测操作模式对免疫人群乙肝核心抗体结果的影响。方法统计2012—2013年用化学发光微粒子免疫检测法(CMIA)和酶联免疫竞争抑制一步法(ELISA)检测乙肝人群的HBsAb及HBcAb阳性率,并按≤2岁、2~20岁、>20岁3个年龄分段比较;收集92例免疫人群样本用CMIA和3种ELISA法检测HBcAb;用同种ELISA法试剂,改变操作模式进行HBcAb检测。结果 3组年龄段检测人群HBsAb两种检测方法统计的阳性率无统计学差异,≤2岁组阳性率最高;HBcAb用CMIA法统计的阳性率在≤2岁组和2~20岁组的免疫人群组低于ELISA法。 92例样本用CMIA法检测HBcAb阳性率为2.2%;用3种ELISA法试剂检测阳性率分别为79.3%、82.6%及94.6%;3种ELISA法试剂检测阳性率无统计学差异。92例样本改变操作模式,用同种ELISA法检测试剂检测结果有19例为阴性,差异有统计学意义。结论检测人群HBsAb检出率在2岁及以下人群最高,随年龄增长抗体下降。 HBcAb用CMIA法检测在免疫人群中检出阳性率明显低于EIISA法。ELISA竞争抑制一步法检测HBcAb易造成假阳性,如改用HBcAb单项加样操作或选用化学发光微粒子免疫检测法(CMIA)检测,可明显减少假阳性。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	张晓琍
	王金龙
	林光华
	曹颖平
	周建林

关键词 ：乙型肝炎病毒表面抗体, 乙型肝炎病毒核心抗体, 抗体检测, 酶联免疫吸附法, 化学发光微粒子免疫检测法

Abstract：In this study, we detected the positive rate of anti-HBs and anti-HBc antibody among the subject population in Fujian Medical University Union Hospital, and to evaluate different detection methods of anti-HBc antibody. The positive rate of anti-HBs and anti-HBc antibody were detected by chemiluminescent microparticle immunoassay (CMIA) and one-step competitive enzyme-linked immunosorbent assay (ELISA) from the year 2012 to 2013. The subject population was divided into three groups: group 1 with the age of less than 2 years old, group 2 with the age of 2-20 years old, and group 3 with the age of more than 20 years old. The positive rates of anti-HBV antibody in the different groups were analyzed. Furthermore, anti-HBc antibody of 92 samples selected from the immunized population was detected by CMIA and three kinds of ELISA reagents. Meanwhile, the detection of anti-HBc antibody by the same ELISA reagent but different operating modes were performed in these samples. The highest positive rate of anti-HBs antibody was detected in group 1, and there was no significance difference of positive rate between two detection methods of anti-HBs antibody among three groups. The positive rate of anti-HBc antibody using CMIA was significantly lower than those with ELISA among group 1 and 2. Among the 92 samples, the positive rate of anti-HBc antibody was 2.2% using CMIA. With three kinds of method of ELISA reagent, the positive rate of anti-HBc antibody were 79.3%, 82.6% and 94.6%, respectively, and there was no statistical significance among the results of three ELISA reagents. Anti-HBc was not detected from 19 samples using ELISA methods with different operating modes. It's concluded that the anti-HBs antibody declined with the increase of age, and it is necessary to discriminate the specific population to strengthen immune system. The obviously higher positive rate of anti-HBc antibody was found by ELISA in immunized population than that by CMIA. Concerning on the false positive of ELISA, specimen sampling with one specific test item or the CMIA method was recommended to detect the anti-HBc antibody.

Key words： anti-HBs antibody anti-HBc antibody antibody detection enzyme-linked immunosorbent assay chemiluminescent microparticle immunoassay

收稿日期: 2013-08-28

PACS:

R446.61

基金资助:国家自然基金项目资助项目(81171656)资助

通讯作者: 曹颖平 E-mail: caoyingping@aliyun.com

引用本文:

张晓琍, 王金龙, 林光华, 曹颖平, 周建林. 免疫人群乙肝抗体阳性率调查及乙肝核心抗体检测方法探讨[J]. 中国人兽共患病学报, 2015, 31(3): 289-292. ZHANG Xiao-li, WANG Jin-long, LIN Guang-hua, CAO Ying-ping, ZHOU Jian-lin. Prevalence of anti-HBV antibody among immunized population and evaluation of different detection methods of anti-HBc antibody. Chinese Journal of Zoonoses, 2015, 31(3): 289-292.

链接本文:

http://www.rsghb.cn/CN/10.3969/cjz.j.issn.1002-2694.2015.03.022 或 http://www.rsghb.cn/CN/Y2015/V31/I3/289

[1] Pan XC. Study on immune persistence of 2 159 children injected with recombinant HBV vaccine[J]. Zhejiang J Prev Med, 2008, 20(9): 16-18. (in Chinese) 潘学春. 2 159名儿童重组乙肝疫苗免疫持久性研究[J]. 浙江预防医学,2008,20(9):16-18.
[2] Liu XL. On the effect of immunization for HBV vaccine[J]. J Dali Univ, 2008, 7(6): 41-42. (in Chinese) 刘鲜丽. 免疫接种乙肝疫苗防治效果探讨[J]. 大理学院学报,2008,7(6):41-42.
[3] Jilg W, Hottentrager B, Weinberger K, et al. Prevalence of markers of hepatitis B in the adult German population[J]. J Med Virol, 2001, 63(2): 96-102.
[4] Chinese Society of Hepatology and Chinese Society of Infectious Diseases Chinese Medical Association. The guideline of prevention and treatment for chronic Hepatitis B (2010 version)[S]. Chin J Hepatol, 2011, 19(1): 13 - 23 . (in Chinese) 中华医学会肝病学分会,中华医学会感染病学分会. 慢性乙型肝炎防治指南(2010版)[S]. 中华肝脏病杂志,2011,19(1):13 - 23 .
[5] Cheng J, Sun CG, Cheng YS, et al. The effects of different operating modes to the competitive ELISA method for detection of anti-HBV[J]. Chin J Clin Lab Sci, 2008, 26(3): 207-208. (in Chinese) 成军,孙长贵,成玉生,等. 不同加样方式对竞争ELISA法检测抗HBc 的影响[J]. 临床检验杂志,2008,26(3):207-208.
[6] Weber B, Melchior W, Gehrke R, et al. Hepatitis B virus markers in anti-HBc only positive individuals[J]. J Med Virol, 2001, 64(3): 312-319.
[7] Cheng J, Xie Y, Wang GZ, et al. Clinical significance of anti-HBc-IgG only positivity of HBV markers in serum[J]. Int J Lab Med, 2006, 27(3): 203-205. (in Chinese) 成军,谢钰,王国政,等. 乙型肝炎血清学标志物单项抗HBc-IgG 阳性结果的解释及临床意义[J]. 国际检验医学杂志,2006,27(3):203-205.
[8] Jiang F, Hu DY. Study the factors of the one-step competitive ELISA method for detection of anti-HBc[J]. West China Med J, 2005, 20(2): 365. (in Chinese) 江帆,胡定远. 酶免疫竞争一步法检测乙型肝炎病毒核心抗体影响因素探讨[J]. 华西医学,2005,20(3):365.