Abstract：We established real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (RT-PCR) for determining the expression of Delta giardin mRNA to explore effects of dihydroartemisinin (DHA) on the expression level of Delta giardin mRNA in C2 Giardia lamblia. Giardia lamblia respectively cultivated for 2, 4, 8, and 12 h respectively with modified TYI-S-33 medium which contained 100 μg/mL and 200 μg/mL DHA, while the negative control group performed in the same experimental condition without DHAs. All of the RNAs were extracted and cDNA were synthesized. The relative expressive quantity of Delta giardin mRNA was determined by real-time PCR and the results were 0.44, 0.26, 0.25, and 0.02 when Giardia lamblia respectively cultivated for 2 h, 4 h, 8 h, and 12 hours which contained 100 μg/mL DHA. The relative expression quantities of Delta giardin mRNA were 0.30, 0.26, 0.11, and 0.02 when Giardia lamblia respectively cultivated for 2 h, 4 h, 8 h, and 12 h which contained 200 μg/mL DHA. The expressive quantities of Delta giardin mRNA with DHA were significantly lower than that in the control group. It suggests that dihydroartemisinin has obvious inhibitory effects on the expression level of Delta giardin mRNA in C2 Giardia lamblia, and DHA has significant prevention and cure function to C2 Giardia lamblia.
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2015, Vol. 31 
