Abstract:We isolated Hantavirus from the rodents' lung samples in order to know the genetic characteristic of new isolates in Jiangxi Province. Samples were collected in 2011, and Hantavirus was isolated through Vero-E6 cells culture, the strains were identified by direct immunofluorescence assay and RT-PCR. The complete S, M segments of one strain were amplified and sequenced, the gene sequences were analyzed with DNAStar and MEGA3.1 software. Two Seoul viruses (SEOV) were isolated and identified from 15 samples. The complete S segment contained 1 772 nucleotides with a single open reading frame coding 429 amino acids; M segment contained 3 651 nucleotides in length with a single open reading frame coding 1 133 amino acids. The similarities to other SEOVs based on S/M nucleotides and amino acid sequences were 94.8%-98.0% and 98.2%-99.6% respectively. Compared to 80-39 strain, there was only one amino acid site changed in the S amino acid sequence and 9 amino acid sites changed in the M amino acid sequence. Results showed that comparing to other SEOVs the genes of new isolates are highly conserved.
刘师文, 徐刚, 施勇, 龚甜, 李健雄, 刘晓庆, 熊英. 江西省汉坦病毒分离及其S和M基因特征分析[J]. 中国人兽共患病学报, 2015, 31(12): 1157-1161.
LIU Shi-wen, XU Gang, SHI Yong, GONG Tian, LI Jian-xiong, LIU Xiao-qing, XIONG Ying. Hantavirus isolation and the complete S, M segments genetic analysis of the isolates in Jiangxi Province, China. Chinese Journal of Zoonoses, 2015, 31(12): 1157-1161.
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