Abstract：The p24 gene fragment of BDV-RNA in brain tissue of bat was detected by nested reverse transcriptase polymerase chain reaction (nRT-PCR) in Zunyi, Guizhou Province. The positive PCR products were analyzed by genetic sequence homology and molecular phylogenetic tree. Result showed that the P24 gene fragment of BDV-RNA in brain tissue from 82 bats was 7 positived. There was only 5 positive products could be successfully detected. The homology of p24gene fragment of BDV was 99%, with 2 situs consistency silent mutation when compared with standard strain V in horse (nt1650T-C, nt1740G-T, mutation rate of 0.9%). The homology of p24 gene fragment of BDV was 97%, with 5 situs consistency silent mutation when compared with H1766 (nt1599A-G, nt1671 T-C, nt1677 T-C, nt1695 G-A, nt1740 G-T, mutation rate of 2.2%). The homology of p24 gene fragment of BDV was 96%, with 7 situs consistency silent mutation when compared with He\80 (nt1566 G-A, nt1581 C-T, nt1659 T-C, nt1668 A-G, nt1674 T-C, nt1695 G-A, nt1740 G-A, mutation rate of 3.1%). There is BDV natural infection probably originated from bat, highly homology with strain V in horse in Zunyi, Guizhou Province.
杨利玲, 雷以会, 徐平. 贵州遵义地区蝙蝠脑组织BDV-P24基因片段的检测[J]. 中国人兽共患病学报, 2016, 32(2): 156-159.
YANG Li-ling, LEI Yi-hui, XU Ping. Borna disease virus p24 in brain tissue of bat infect in Zunyi, Guizhou Province, China. Chinese Journal of Zoonoses, 2016, 32(2): 156-159.
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2016, Vol. 32 
