Abstract：In order to detect Schistosoma japonicum circulating antigen and to analyze the change of antibody in host,gold nanorods were prepared by seed-mediated growth and were deposited onto the ITO-glass slide. SIEA26-28k DaSjscFv antibody was bound onto the solid phase sensor and identified from the longitudinal plasmon of the GNRs wavelength shift as the optical signature. The biosensor was used to detect S.japonicum circulating antigens by employing Local Surface Plasmon Resonance (LSPR) changes,which subsequently resulted in better optical recognition of antigen-antibody binding. We demonstrate that the biosensor could accurately distinguish between S.japonicum-positive and -negative serum samples under the same conditions. We detected the infection serum with S.japonicum in different periods (1 to 8 week,respectively),and the results showed the different red shift was 17 nm,52 nm,28 nm,11 nm,13 nm,23 nm,45 nm and 43 nm respectively,and the change rule of antibody can be provided by these data. Meanwhile,the sensor,with high sensitivity and specificity,can offer a new monitoring method to study the antigen-antibody and diagnosis of schistosomiasis,and also bring new ideas for other parasite prevention and treatment.
何鑫, 汪世平, 周云飞, 黄成铭, 闾丘思嘉, 宁水兵. 金纳米棒免疫传感器检测不同感染周期日本血吸虫循环抗原的研究[J]. 中国人兽共患病学报, 2016, 32(4): 315-320.
HE Xin, WANG Shi-ping, ZHOU Yun-fei, HUANG Cheng-ming, LYUQIU Si-jia, NING Shui-bing. Detection on Schistosoma japonicum circulating antigen from rabbit serum in different periods using gold nanorod biosensors. Chinese Journal of Zoonoses, 2016, 32(4): 315-320.
[1] Dang H,Xu J,Li SZ,et al. Monitoring the transmission of Schistosoma japonicum in potential risk regions of China,2008-2012[J]. Int J Environ Res Public Health,2014,11(2): 2278-2287. doi:10.3390/ijerph110202278 [2] Chitsulo L,Engels D,Montresor A,et al. The global status of schistosomiasis and its control[J]. Acta Trop,2000,77(1): 41-51. doi:10.1016/S0001-706X(00)00122-4 [3] Chitsulo L,Loverde P,Engels D. Schistosomiasis[J]. Nat Rev Microbiol,2004,2(1): 12-13. doi:10.1038/nrmicro801 [4] Kongs A,Marks G,Verle P,et al. The unreliability of the Kato-Katz technique limits its usefulness for evaluating S.mansoni infections[J]. Trop Med Int Health,2001,6(3): 163-169. doi:10.1046/j.1365-3156.2001.00687.x [5] Wen ZL,Wang SP,Wu ZY,et al. A novel liquid-phase piezoelectric immunosensor for detecting Schistosoma japonicum circulating antigen[J]. Parasitol Int,2011,60(3): 301-306. doi:10.1016/j.parint.2011.05.001 [6] Zhang XE. Biosensor[M]. Beijing: Chemical Industry Press,2006: 1-15. (in Chinese) 张先恩.生物传感器[M].北京:化学工业出版社,2006:1-15. [7] Lakhani PM,Rompicharla SV,Ghosh B,et al. An overview of synthetic strategies and current applications of gold nanorods in cancer treatment[J]. Nanotechnology,2015,26 (43): 432001. doi:10.1088/0957-4484/26/43/432001 [8] Che HL,Wang SP,Wu ZM,et al. A new fluorescence immunoassay based on magnetic nanoparticles for Schistosona japonicum antibodies[J]. Chin J Analytical Chem,2008,36(11): 1455-1459. (in Chinese) 车宏莉,汪世平,吴争鸣,等. 基于磁性纳米颗粒的日本血吸虫抗体荧光免疫分析[J].分析化学,2008,36(11):1455-1459. [9] Chen CD,Cheng SF,Chau LK,et al. Sensing capability of the localized surface plasmon resonance of gold nanorods[J]. Biosens Bioelectron,2007,22(6): 926-932. doi:10.1016/j.bios.2006.03.021 [10] Huang H,Liu F,Huang S,et al. Sensitive and simultaneous detection of different disease markers using multiplexed gold nanorods[J]. Anal Chim Acta,2012,755: 108-114. doi:10.1016/j.aca.2012.10.020 [11] Jain PK,Eustis S,El-Sayed MA. Plasmon coupling in nanorod assemblies: optical absorption,discrete dipole approximation simulation,and exciton-coupling model[J]. J Phys Chem B,2006,110(37): 18243-18253. doi:10.1021/jp063879z [12] Sau TK,Murphy CJ. Self-assembly patterns formed upon solvent evaporation of aqueous cetyltrimethylammonium bromide-coated gold nanoparticles of various shapes[J]. Langmuir,2005,21(7): 2923-2929. doi:10.1021/la047488s [13] Nash TE. Factors that modulate clearance and ultimate fate of a specific schistosome antigen (GASP) in schistosome infections[J]. J Immunol,1982,128(4): 1608-1613. [14] Nash TE. Fate and mechanism of clearance of PSAP,a schistosome antigen,in schistosomiasis[J]. J Immunol,1983,131(5): 2520-2523. [15] Liu SG,Song L,Xie LP,et al. Dynamic observation on the relationship between Schistosoma japonicum infectiosity and ITS titers of circulating antigens detected with Dot-ELISA[J]. Chin J Parasit Dis Ctrl,1998,11(3): 204-206. (in Chinese) 刘世国,宋力,谢来平,等.应用Dot-ELISA进行日本血吸虫循环抗原检测时其滴度与感染度关系的动态观察[J].中国寄生虫病防治杂志,1998,11(3):204-206.
2016, Vol. 32 
