动物布鲁氏菌病快速诊断方法研究进展

doi:10.3969/j.issn.1002-2694.2016.08.012

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (722 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要布鲁氏菌病是一种流行范围广、危害严重的人畜共患传染病。近年来,布鲁氏菌病的人畜疫情在国内外均出现回升势头,严重影响了流行地区的养殖产业发展,并引发严重的公共卫生问题。因此,该病的快速准确检测能为预防、治疗提供早期、有效的疫病信息。鉴于布鲁氏菌病危害的严重性,建立快速有效的检测方法是十分必要的,是布鲁氏菌病早期诊断和疾病控制的关键。本文将对多重PCR、实时定量荧光PCR、LAMP等分子生物学检测方法以及ELISA、胶体金试纸条等免疫学检测方法两个方面对布鲁氏菌病快速检测方法的研究进展进行综述。其中LAMP方法和胶体金免疫层析法对布病的检测更加适合在基层的推广和使用,并且免疫层析技术正朝着定量、高灵敏度、多标记检测等方向发展。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	吴彤
	王慧煜
	吴绍亮
	崔金磊
	杨晓野
	吴绍强

关键词 ：布鲁氏菌病, 多重PCR, 荧光PCR, 环介导等温扩增反应, 酶联免疫吸附试验, 胶体金试纸条, 诊断

Abstract：Brucellosis is a serious zoonosis which is widely prevalent in the world. Recently, the morbidity of human and animal brucellosis has threatened the development of animal husbandry and the safety of public health. Prompt and accurate diagnosis for brucellosis plays a key role in disease control and eradication. The molecular biological method such as multiplex PCR, real-time quantitative PCR, LAMP and the immunological method including ELISA and GICA, used in the rapid brucellosis diagnosis is reviewed in this article.It is indicated that the lamp method and colloidal gold immunochromatography detection of brucellosis is more suitable for the promotion and use in basic and in immunochromatographic technique for quantitative, high sensitivity, multi marker detection.

Key words： brucellosis multiplex PCR real time quantitative PCR LAMP ELISA GICA diagnosis

收稿日期: 2016-03-09

PACS:

R378

基金资助:国家质检公益性行业科研专项基金(No.201510017)资助

通讯作者: 杨晓野,Email:xiaoyeyang122@sohu.com

作者简介: 杨晓野,Email:xiaoyeyang122@sohu.com

引用本文:

吴彤, 王慧煜, 吴绍亮, 崔金磊, 杨晓野, 吴绍强. 动物布鲁氏菌病快速诊断方法研究进展[J]. 中国人兽共患病学报, 2016, 32(8): 746-751. WU Tong, WANG Hui-Yu, WU Shao-liang, CUI Jin-lei, YANG Xiao-ye, WU Shao-qiang. Research progress on rapid diagnosis of animal brucellosis. Chinese Journal of Zoonoses, 2016, 32(8): 746-751.

链接本文:

http://www.rsghb.cn/CN/10.3969/j.issn.1002-2694.2016.08.012 或 http://www.rsghb.cn/CN/Y2016/V32/I8/746

[1] Gao MH,Zhang ZY,Li Y,et al. The research progress of laboratory diagnosis of brucellosis[J]. Chin J Zoonoses,2010,26(1):81-83.DOI:1002-2694(2010)01-0081-03.(in Chinese)
高明华,张志琰,李跃,等.布鲁氏菌病实验室诊断研究进展[J].中国人兽共患病学报,2010,26(1):81-83. DOI:1002-2694(2010)01-0081-03
[2] Du XY,Chen ZL,Wang YF,et al. Establishment of a multiplex PCR for Brucella detection [J]. J Prevent Med PLA,2008,26(5) :341-343.doi:10.13704/j.cnki.jyyx.2008.05.009(in Chinese"> doi:10.13704/j.cnki.jyyx.2008.05.009(in Chinese) 杜昕颖,陈泽良,王玉飞,等.多重PCR特异检测布鲁氏菌方法的建立[J].解放军预防医学杂志,2008, 26(5) : 341-343. doi:10.13704/j.cnki.jyyx.2008.05.009
[3] Zhang SD. The establishment and application of multiplex PCR method and indirect ELISA method for the detection of Brucella [D].Yang Lin:North West Agriculture and Forestry University,2011.(in Chinese) 张三东.布鲁氏菌多重PCR及间接ELISA检测方法的建立与应用[D].扬林:西北农林科技大学,2011.
[4] Chen J,Cui BY,Zhao HY,et al. Study on the application of multiple PCR identification of Brucella strain and pig 5 biotypes[J]. Journal of Pathogen Biology,2013,8(2) :126-130.doi:10.13350/.cjpb.2013.02.003.(in Chinese"> doi:10.13350/.cjpb.2013.02.003.(in Chinese) 陈军,崔步云,赵鸿雁,等.应用多重PCR鉴别布鲁氏菌种及猪种5个生物型研究[J].中国病原生物学杂志,2013,8(2):126-130. doi:10.13350/.cjpb.2013.02.003
[5] Chen S,Wang XR,Qian J,et al. Cattle, sheep, pigs, establish a multiplex PCR method for canine brucellosis[J]. Chin Husband,Vet Med,2014,42(2) :29-34.DOI:1671-7236(2014)02-0029-06.(in Chinese) 陈思,王秀然,钱晶,等.牛、羊、猪、犬布鲁氏菌病多重PCR方法的建立[J].中国畜牧兽医,2014,42(2):29-34. DOI:1671-7236(2014)02-0029-06
[6] Xie ZX,Xie ZQ,Liu JB,et al. Research and application of rapid detection and identification of Brucella mycobacterium bovis by multiplex PCR[J].Chinese Journal of Zoonoses,2007,23(7) :714-717.DOI:1002-2694(2007)-07-0714-04(in Chinese) 谢芝勋,谢志勤,刘加波,等.多重PCR快速检测鉴别牛布鲁氏菌和牛分枝杆菌的研究与应用[J].中国人兽共患病学报,2007,23(7):714-717. DOI:1002-2694(2007)-07-0714-04
[7] Zeng QH. A preliminary study on the establishment and clinical application of real-time PCR assay for rapid detection of Brucella[D].Changchun:Jilin University,2014.(in Chinese) 曾庆贺.布鲁氏菌实时荧光定量PCR快速检测方法的建立和临床应用的初步研究[D].长春:吉林大学,2014
[8] Li GH. The assembly to establish fluorescence quantitative PCR for rapid detection of Brucella and detection kit[D].Changchun:Jilin University,2006.(in Chinese) 李光辉.布鲁氏菌荧光定量PCR快速检测方法的建立及检测试剂盒的组装[D].长春:吉林大学, 2006
[9] Meng R. Preliminary study on rapid diagnostic test for Brucella real-time fluorescent quantitative detection method and serum[D]. Shihezi:Shihezi University,2010.(in Chinese) 梦茹.布鲁氏菌实时荧光定量检测方法与血清快速诊断试纸的初步研究[D].石河子:石河子大学, 2010. 1-57.
[10] Gao ZQ,Xing J,Feng YF,et al. Rapid detection of Brucella TaqMan MGB probe real-time fluorescence quantitative PCR[J]. Chinese Journal of Zoonoses,2011,27(11):995-1000.DOI:1002-2694(2011)11-0995-06.(in Chinese) 高正琴,邢进,冯育芳,等.TaqMan MGB探针实时荧光定量PCR快速检测布鲁氏菌[J].中国人兽共患病学报,2011,27(11):995-1000. DOI:1002-2694(2011)11-0995-06
[11] Dahouk S,Tomaso H,N ckler K,et al.The detection of Brucella spp.Using PCR-ELISA and real-time PCR assays[J].Clinical Laboratory,2004,50:387-394.
[12] Ma GR. Loop mediated isothermal amplification (LAMP) method for detection of Brucella pathogen in the northern region of Xinjiang[D]. Shihezi:Shihezi University,2014.(in Chinese) 马国瑞.环介导等温扩增(LAMP)检测新疆北疆地区布鲁氏菌病原的方法研究[D].石河子:石河子大学,2014
[13] Pan W,Li SS,Zhao MQ,et al. Establishment and application of a new method of detection of Brucella LAMP visualization[R]. Guangzhou:South China Agricultural University,20 10,336-341.(in Chinese) 潘文,李珊珊,赵明秋,等.新型可视化LAMP检测布鲁氏菌方法的建立及初步应用[R].广州:华南农业大学, 2010,336-341.
[14] Lin GZ. Study on molecular vaccine co expressing a Brucella LAMP detection method and the double gene[D].Lanzhou:Lanzhou Veterinary Institute,2012,1-88.(in Chinese) 蔺国珍.布鲁氏菌病LAMP检测方法的建立及双基因共表达分子疫苗研究[D]. 兰州:兰州兽医研究所,2012,1-88.
[15] Xu ZL,Nan WL,Zhou J,et al. Brucella loop mediated isothermal amplification (LAMP) based on the visual detection method[J].Chinese Anin Health Inspection,2011,28(8): 37-40.DOI:1005-944X(2011)08-0037-04.(in Chinese)
许邹亮,南文龙,周杰,等.布鲁氏菌环介导等温扩增(LAMP)可视化检测方法的建立[J].中国动物检疫,2011,28(8):37-40. DOI:1005-944X(2011)08-0037-04.
[16] Ohtsuki R,Kawamoto K,Kato Y,et al. Rapid detection of Brucella spp. by the loop-mediated isothermal amplification method[J]. J Appl Microbiology,2008,104(6): 1815-1823,doi:10.1021/jp810387w
[17] Zhang L. Loop mediated isothermal amplification (LAMP) is established and compared with the method of quantitative detection of Brucella [D].Baotou: Inner Mongolia Agricultural University,2012,1-54.(in Chinese)
张利. 环介导等温扩增技术(LAMP)与荧光定量检测布鲁菌方法的建立及对比试验[D].包头:内蒙古农业大学,2012,1-54.
[18] Garin -Bastuji B,Blasco JM,Grayon M,et al. Brucella melitensis infection in sheep:present and future[J]. Vet Res,1998,29(3-4):255-274.
[19] Zuo YZ,Wang ZL,Lu GJ,et al. The establishment of Brucella BP26 indirect ELISA detection method[J]. ChineseJournal of Preventive Veterinary Medicine,2014,36(3):223-226.doi:10.3969/j.issn.1008-0589.2014.03.13.(in Chinese"> doi:10.3969/j.issn.1008-0589.2014.03.13.(in Chinese)
左玉柱,王增利,路广计,等.牛布鲁氏菌BP26间接ELISA检测方法的建立[J].中国预防兽医学报,2014,36(3):223-226. doi:10.3969/j.issn.1008-0589.2014.03.13 [20] Jia JF,Zhang Q. Study and application of double antibody sandwich ELISA method for detection of Brucella antigen[J].Contemporary Medicine,2009,15(24):87-89.(in Chinese) 贾剑峰,张琴.双抗夹心ELISA方法检测人布鲁氏菌抗原的研究及初步应用[J].当代医学,2009,15(24):87-89.
[21] Wang ZM,Zhao N,Wang F,et al. Establishment and development of cELISA method for anti Brucella monoclonal antibody[J]. Veterinary Science in China,2014, 44(9):909-91413.DOI:1673-4696(2014)09-0909-06.(in Chinese) 王志明,赵妮,王芳,等.抗布鲁氏菌单克隆抗体的研制及cELISA方法的建立[J].中国兽医科学,2014,44(9):909-91413. DOI: 1673-4696(2014)09-0909-06.
[22] Zhang H,Chen CF,Qiao J,et al. Establishment and preliminary application of indirect ELISA method for detection of Brucella recombinant protein based on SP41[J].Chinese Acta of veterinary,2010,30(5): 624-62.DOI:1005-4545(2010)05-0624-05.(in Chinese) 张辉,陈创夫,乔军,等.基于重组蛋白SP41的布鲁氏菌间接ELISA检测方法的建立及初步应用[J].中国兽医学报,2010,30(5):624-62. DOI:1005-4545(2010)05-0624-05
[23] Chen TT,Shi F,Chen CF,et al. The establishment of rapid detection of Brucella antibody colloidal gold immunochromatography[J]. Journal of Pathogen Biology,2014, 9(2):122-126.DOI:1673-5234(2014)02-0122-05(in Chinese) 程婷婷,石峰,陈创夫,等.布鲁氏菌抗体胶体金免疫层析法快速检测技术的建立[J].中国病原生物学杂志,2014,9(2):122-126. DOI:1673-5234(2014)02-0122-05
[24] Zhu MD,Hong LD. Study on rapid diagnosis of bovine brucellosis colloidal gold immunochromatography[J]. Chinese Journal of Zoonoses,2008,24(8):755-759.DOI:1002-2694(2008)08-0755-02(in Chinese) 朱明东,洪林娣.胶体金免疫层析法快速诊断牛布鲁氏菌病的研究[J].中国人兽共患病学报,2008,24(8):755-759.
[25] Zhang FX,Li XY,Yan GM,et al. With the development of natural infection animal vaccine immune colloidal gold test strip animal brucellosis identification[J]. Chin Husbandry Vet Med,2009,36(7):79-82.DOI:1671-7236(2009)07-0079-04(in Chinese) 张付贤,李晓艳,闫广谋,等.鉴别布鲁菌病自然感染动物与疫苗免疫动物胶体金检测试纸条的研制[J].中国畜牧兽医,2009,36(7):79-82.
[26] Tlu Z,Elu M,Al K,et al.Use of specific antibodies,labeled with colloidal gold particles,for the detection of Brucella antigens using dot-Immunoassay[J]. Zhurnal Mikrobiologii,Epidemiologii,Iimmunobiologii,1998,(6):64-68.
[27] Ismail TF,Smits H,Wasfy MO,et al.Evaluation of dipstick serologic tests for diagnosis of brucellosis and typhoid fever in Egypt[J].Journal of Clinical Microbiology,2002,4 0(9):3509-3511.
[28] Clavijo E,Díaz R,Anguita A,et al.Comparison of a dipstick assay for detection of Brucella -specific immunoglobulin M antibodies with other tests for serodiagnosis of human brucellosis[J].Clin Diagn Lab Immunol,2003,10(4):612-615.
[29] Ren ZQ,Wu YS,Liu TC,et al.Research progress of new type of immune chromatography[J].Guangdong Med J,2013,34(2):312-331.(in Chinese) 任志奇,吴英松,刘天才,等.新型免疫层析技术的研究进展[J].广东医学,2013,34(2):312-331.