Establishment of secondary hydatid disease infection in mice with cystic and alveolar Echinococcus cysts cultured in vitro
WANG Hui, LI Jun, GUO Bao-ping, WEN Hao, ZHANG Wen-bao
State Key Laboratory Incubation Base of Xinjiang Major Diseases Research,Clinical Medical Research Institute, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China
Abstract:We established a stable mice model for cystic and alveolar echinococcosis (CE and AE) infection. E. granulosus (Eg) protoscoleces were aspirated and pooled from sheep liver hydatid cysts collected from a slaughterhouse in Urumqi. E. multilocularis (Em) protoscoleces were aspirated from hamster rats infected with protoscoleces of Em. Pepsin digested protoscoleces of two species of Echinococcus were cultured to small cysts. BALB/c mice were each intraperitoneally (i.p) transplanted with 50 small cultured cysts of Eg or Em. Hydatid cysts were collected from the mice and processed for histopathological examination by HE staining after six month of transplantation infection. To evaluate the success of the established animal model for infection with Eg and Em, the mice were sacrificed and the cyst number in each of the mice was counted. After 60 days cultured in vitro, we found that protoscoleces of Eg and Em were differentiated into small cysts and Eg cysts had a thicker cell free laminated layer compared to the laminated layer of Em cysts. All the mice were infected successfully; the infection rates were 100%. Eg hydatid cysts formed many single capsules in the peritoneal cavity of mice and we obtained a recovery rate (viability) of 70% in term of success of transplanted small cysts. However, Em hydatid cysts generated cell mass from the germinal layer which then formed brood capsules and produced protoscoleces observed in the small brood capsules. The process of in vitro cultured protoscoleces of Echinococcus remarkably increases the success of established cysts in mice.
王慧, 李军, 郭宝平, 温浩, 张文宝. 微囊法棘球蚴继发感染小鼠动物模型的建立[J]. 中国人兽共患病学报, 2016, 32(9): 784-788.
WANG Hui, LI Jun, GUO Bao-ping, WEN Hao, ZHANG Wen-bao. Establishment of secondary hydatid disease infection in mice with cystic and alveolar Echinococcus cysts cultured in vitro. Chinese Journal of Zoonoses, 2016, 32(9): 784-788.
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