Function of an internalin gene NTSN_0462 in Listeria monocytognes serotype 4b
ZHAO Dan, YAO Hao, CAI Xue-xue, KONG Su-wei, YUN-Xi, TAN Wei-jun, PAN Zhi-ming, YIN Yue-lan, JIAO Xin-an
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Joint International Research Laboratory of Agriculture Agri-Product Safety, Yangzhou 225009, China
Abstract：Internalins play a key role in the adhesion and invasion of L.monocytogenes into host cells. In this study, the function of internalin gene NTSN_0462 in serotype 4b Lm NTSN was probed into. Firstly, NTSN_0462 was knocked out by homology recombination, then the pathogenic characterization of mutant strain LmNTSNΔ0462 was investigated in vivo and in vitro. The growth curve showed that the deletion of NTSN_0462 gene didn?t affect growth and metabolism of Lm in BHI medium. The invasion of the mutant strain into human intestinal epithelial cell line Caco-2 and human hepatocyte cell line HepG2 significantly reduced comparing with the WT strains (P<0.001). Furthermore, the infection ability to BALB/c mouse was analyzed in vivo, compared with the WT strains, the colonization of LmNTSNΔ0462 in liver decreased significantly (P<0.001), wheareas there was no significant difference in the spleen, indicating that NTSN_0462 plays an important role in the invasion and multiplication in host livers, heretofore it is an invasion-related virulent factor of L.monocytogenes.
赵丹, 姚浩, 蔡雪薛, 孔苏伟, 恽茜, 谈卫军, 潘志明, 殷月兰, 焦新安. 4b型单核细胞增生李斯特菌内化素基因NTSN_0462的功能初探[J]. 中国人兽共患病学报, 2017, 33(2): 115-119.
ZHAO Dan, YAO Hao, CAI Xue-xue, KONG Su-wei, YUN-Xi, TAN Wei-jun, PAN Zhi-ming, YIN Yue-lan, JIAO Xin-an. Function of an internalin gene NTSN_0462 in Listeria monocytognes serotype 4b. Chinese Journal of Zoonoses, 2017, 33(2): 115-119.
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2017, Vol. 33 
