Molecular characteristics of Listeria monocytogenes foodborne isolates in Hangzhou, China
YU Hua1, 2, PAN Jing-cao2, WANG Hao-qiu2, SI Guo-jing2, LIU Tao2, LOU Xiu-qin2, ZHANG Wei2, YAN Jie1
1. Department of Medical Microbiology and Parasitology, School of Medicine,Zhejiang University, Hangzhou 310058, China; 2. Hangzhou Center for Disease Control and Prevention, Hangzhou 310026, China
Abstract：We determined molecular characteristics of Listeria monocytogenes foodborne isolates in Hangzhou and investigated the characterization of local strains. Multi-locus sequence typing(MLST) and pulsed-field gel electrophoresis(PFGE) were applied to identify molecular types of Listeria monocytogenes isolates. Results showed that a total of 133 strains of 6 serotypes were divided into 19 MLST types including a new type ST767. ST9 and ST121 were the major ST types. There were 33 and 45 PFGE patterns characterized by AscI and ApaI. The molecular types of Listeria monocytogenes strains were widely distributed in Hangzhou. It is indicated that the major clusters were Lineage I and Lineage II which will cause listeriosis. The contamination of Listeria monocytogenes in food is serious in Hangzhou and the surveillance and management should be strengthened to prevent the food borne diseases.
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2017, Vol. 33 
