Epidemiological characteristics investigation of enteropathogenic Escherichia coli in diarrhea patients from Kunming City, China
YANG Chun-li1, ZHANG Shun-xian1, AI Lin1, CAI Yu-chun1, LU Yan1, CHEN Shao-hong1, XU Wen2, GU Wen-peng2, HU Wei1, 3, CHEN Jun-hu1, LI Shi-Zhu1, CHEN Jia-xu1, ZHOU Xiao-nong1
1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory for Parasitology and Vector Biology, MOH of China, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, China; 2. Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China; 3. Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China
Abstract：In order to provide scientific reference for the prevention and treatment of EPEC infection, the epidemiological characteristics of enteropathogenic Escherichia coli(EPEC) was investigated in Kunming City, Yunnan. A total of 1 121 diarrhea patients and 319 healthy controls were recruited from four sentinel hospitals in Kunming City from June 2014 to July 2015. The stool samples were cultured and the total DNA was extracted to detect EPEC with real-time polymerase chain reaction (PCR). A structured questionnaire was applied to each case for the clinical manifestation and the basic information. Results showed that the detection rate of EPEC in diarrhea patients was more than the controls (5.53%, 1.88%, χ2=7.36, P<0.05). The prevalence of EPEC was no significant difference between male and female in diarrhea patients (5.54%, 5.53%, χ2=0.001, P=0.999). However, the detection rate of EPEC had significant difference in different age groups (χ2=17.27, P<0.05), among which the detection rate was the highest in 2—5 years group (12.12%, 16/132). And the prevalence of EPEC showed remarkable seasonal trend in diarrhea cases (χ2=12.82, P<0.05), and the detection rate was the highest in summer (9.00%, 18/200) and the lowest in winner (3.05%, 12/394). Watery stool was found in 61.29% (38/62) in diarrhea cases with EPEC infection, and dehydration was found in 12.9% (8/62) in diarrhea cases associated with EPEC infection. Results showed EPEC is one of the mainly pathogens causing diarrhea in Kunming City, and EPEC infection occurred mainly in children under 5 years, and it is prone to dehydration.
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2017, Vol. 33 
