Identification of Escherichia albertii from healthy carriers in Zigong, Sichuan Province, China
ZHANG Ling1, LIU Xiang1, XU Yan-mei2, LI Xin-qiong2, WANG Hong1, XIONG Yan-wen2
1.Zigong Center for Disease Control and Prevention, Zigong 643000,China; 2. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206,China
Abstract:We investigated the carrying situation of Escherichia albertii from healthy people engaged in breeding and slaughtering poultry for a long time. We collected stool samples from people engaged in breeding and slaughtering poultry and other healthy people. After enriched with EC broth, eae-positive enrichment culture was directly streaked on MacConkey, and eae-positive lactose non-fermenting isolate was retained for further investigation. The 16S rDNA sequencing and multilocus sequence typing (MLST) were applied in the identification of E. albertii from suspected strains. Intimin subtypes and cdtB types of E. albertii strains were detected. Pulsed-field gel electrophoresis (PFGE) was used to detected genetic polymorphism of strains from this study and animal source ones. Results showed that two isolates were identified as E. albertii from 189 stools of people exposed to slaughtering chickens and ducks and one from 58 stools in control groups. No isolate was identified as E. albertii from 138 stools samples of people exposed to breeding poultry. Intimin subtypes of three isolates from stool samples were subtyped as sigma, iota 2, nu, and cdtB types were closely related to types II/III/V. PFGE patterns of the three strains was distinguishable (<80% similarity), and appeared in different cluster with chickens, ducks and other sources of E. albertii strains. The rate of carrying E.albertii to a certain extent exist in healthy people engaged in slaughtering chickens and ducks, and the relationship between these strains and strains from poultry should be further investigated.
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