Salmonella virulence gene analysis in poisoning food, slaughtering and commercial samples
NIU Li-ya1, XU Bao-hong2, CAI Wen-hua3, WANG Yan1, YANG Xin-ying1, GUO Yu-mei2, SUN Dian-xing1
1. Department of Infectious and Liver disease, Bethune International Peace Hospital, Shijiazhuang 050082, China; 2. Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang 050011, China; 3. Emergency Center, Hebei Airport Management Group Co., Ltd., Shijiazhuang 050802, China
Abstract:We investigated the carrying status of the virulence genes of Salmonella from different sources in Shijiazhuang City, China, to provide the basic data for the further risk assessment of Salmonella. A total of 186 isolates of Salmonella from different sources were collected and identified serotypes in the area of Shijiazhuang from 2011 to 2016. PCR was performed for eight virulence genes (invA, sopE, agfA, spvR, hilA, stn, pefA, shdA). These Salmonella bacteria were detected in 13 kinds of serotypes. Enteritidis is a significant advantage of the group. The above 8 virulence genes were analyzed, and the virulence genes hilA, stn and invA were the most frequently carried, their respective carrying rate were 90.3% (168/186), 86.6% (161/186) and 82.8% (154/186) respectively. We found the virulence genes of Salmonella from different sources were different. It is necessary to take measures to strengthen the food hygiene supervision and prevention and control of the storage and sale of raw poultry stalls in the morning market in Shijiazhuang area.
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