Abstract:We investigated the complete genome characteristic of the Hantaan virus strain AYW89-15 isolated in Jiangxi Province, China. Primers were designed and were used to amplify the complete S, M and L segments by RT-PCR. The PCR product were then cloned and sequenced, the gene sequences were analyzed with DNAStar and MEGA6.0 software. Result showed that the complete gene sequence was 11 848 nucleotides in length, the S, M and L segments were 1 699 nt, 3 616 nt and 6 533 nt respectively, encoding 429, 1 133 and 2 151 amino acids respectively. The sequence identities between stain AYW89-15 and other Hantaan virus were 79.7%-87.3% at the nucleotide level and 92.3%-98.4% at the amino acid level. Phylogenetic analysis of HV showed AYW89-15 belonged to a new HTNV lineage. AYW89-15 was a new subtype of HTNV that exists in Jiangxi Province.
[1] Vaheri A, Strandin T, Hepojoki J, et al. Uncovering the mysteries of hantavirus infections[J]. Nat Rev Microbiol, 2013, 11(8): 539-550. [2] Castel G,Tordo N,Plyusnin A. Estimation of main diversification time-points of hantaviruses using phylogenetic analyses of complete genomes[J]. Virus Res, 2017, 233(2): 60-69. [3] Holmes EC, Zhang Y. The evolution and emergence of hantaviruses[J]. Curr Opin Virol, 2015, 10(10): 27-33. [4] Wang SW, Hang CS, Wang H, et, al. Genotype and clade distribution of Hantaviruses in China[J]. Chin J Virol, 2002, 18(3): 211-216. (in Chinese) 王世文,杭长寿,王华,等.我国汉坦病毒基因型和基因亚型的分布研究[J].病毒学报, 2002, 18 (3):211-216. [5] Liu SW, Gong T, Shi Y, et al. Isolation and characterization of a Hantaan virus in Jiangxi Province[J]. Mod Prevent Med, 2016, (18): 3394-3397. (in Chinese) 刘师文,龚甜,施勇,等.江西省1株汉滩型汉坦病毒的分离与鉴定[J].现代预防医学, 2016(18): 3394-3397. [6] Liu SW, Xu G, Gong T, et, al. Genetic characteristic analysis of Hantavirus from rodents in Jiangxi province[J]. Chin J Vector Bio Ctrl, 2015, 26(5): 475-479. (in Chinese) 刘师文,徐刚,龚甜,等.江西省鼠类携带汉坦病毒基因特征研究[J].中国媒介生物学及控制杂志, 2015, 26(5).475-479. [7] Papa A, Vaheri A, LeDuc JW, et al. Meeting report: Tenth International Conference on Hantaviruses[J]. Antiviral Res, 2016. doi:10.1016/j.antiviral. 2016.08.015 [8] Liu X, Zhang T, Xie C, et al. Changes of HFRS incidence caused by vaccine intervention in Yichun City, China, 2005-2013[J]. Med Sci Mon, 2015, 22: 295-301. [9] Liu SW, Xu G, Shi Y, et, al. Hantavirus isolation and the complete S,M segments genetic analysis of the isolates in Jiangxi province[J].Chin J Zoonoses, 2015,31(12):1157-1162. doi:10.3969/j.issn.1002-2694.2015.12.014(in Chinese) 刘师文,徐刚,施勇,等.江西省汉坦病毒分离及其S和M基因特征分析[J].中国人兽共患病学报,2015,31(12),1157-1162. [10] Straková P, Pdufkova L, Širmarová J, et, al, Novel hantavirus identified in European bat species Nyctalus noctula [J]. Infect Genet Evol, 2017, 48: 127-130. [11] Zhou JH, Zhang HL. Advances in hantavirus typing and pathogenicity[J]. Intl J Virol, 2008, 25(2): 37-41. (in Chinese) 周济华,张海林.汉坦病毒分型及致病性的研究进展[J].国际病毒学杂志, 2008, 15(2).37-41. [12] Zhang YZ, Qu YG, Yang DM. The genotypes and phylogenetic analysis of Hantavirus[J]. Chin J Zoonoses, 2006, 22(3): 193-197. (in Chinese) 张永振,屈勇刚,杨东明,等.汉坦病毒系统发生分析及其基因分型的研究[J].中国人兽共患病学报, 2006, 22(3):193-197. [13] Xu L, Wu J, He B, et al. Novel hantavirus identified in black-bearded tomb bats, China[J]. Infect Genet Evol, 2015, 31: 158-160. [14] Li JL, Ling JX, Liu DY, et al. Genetic characterization of a new subtype of Hantaan virus isolated from a hemorrhagic fever with renal syndrome (HFRS) epidemic area in Hubei Province, China[J]. Arch Virol, 2012, 157(10): 1981-1987. [15] Elgh F, Ke L, Alexeyev OA, et al. A major antigenic domain for the human humoral response to Puumala virus nucleocapsid protein is located at the amino-terminus[J]. J Virol Methods,1996, 59(1/2): 161-172.