Identification of two new sequence type of Brucella isolates by multilocus sequence typing
LYU Yan-ning1, 2, GUO Xiao-xiao2, CHEN Li-juan1, LYU Bing1, CHANG Jian-hua3, HUA Wen-hao4, WANG Hui-zhu4, LI Xin-yu1, WANG Quan-yi1
1. Beijing Center for Diseases Prevention and Control, Beijing Research Centre for Preventive Medicine, Beijing 100013, China; 2. School of Public Health, Capital Medical University, Beijing 100069, China; 3. Pinggu District Center for Diseases Prevention and Control, Beijing 101200, China; 4. Beijing Di Tan Hospital Affiliated of Capital Medical University, Beijing 100015, China
Abstract:We defined the sequence type (ST) of 2 Brucella strains isolated from patients’ blood in Beijing by multilocus sequence typing (MLST) and investigate their genetic characteristics to provide scientific basis for the control and prevention of brucellosis. The 2 isolates were identified using Brucella genus specific BCSP31-PCR and species-specific AMOS-PCR. Nineteen house-keeping genes, one out membrane protein gene and one gene-inter-region sequence of the strains were further analyzed using MLST. The sequences were compared with the existing allele sequences on the MLST website to determine the allelic profile and sequence type and to determine the 9-loci ST and 21-loci ST. Clustering analysis was used to investigate the genetic relationship between the 2 new STs and other known STs of Brucella. The 2 isolates were identified as Brucella with BCSP31-PCR. AMOS-PCR results suggested that the isolates were not B. melitensis, not type 1, 2 and 4 B. abortus, not type 1 B. suis, and not B. ovis. MLST analysis revealed that 2 novel allele combinations (gap/aroA/glk/dnaK/gyrB/trpE/cobQ/int-hyp/omp25/prpE/caiA/csdB/soxA/leuA/mviM/fumC/fbaA/ddlA/putA/mutL/acnA) 2, 1, 2, 2, 1, 3, 1, 4, 1, 13, 2, 2, 2, 2, 1, 1, 3, 7, 6, 2, 3 and 2, 1, 2, 2, 1, 3, 1, 4, 29, 13, 2, 2, 2, 2, 1, 1, 3, 7, 6, 2, 3 were found in the isolates but not reported before. Clustering analysis revealed that the 2 novel STs located at the same branches as the STs of B. abortus. The 2 isolates from Beijing were identified as 2 novel STs by this study which were confirmed by the MLST website,and were assigned as ST74 and ST75 (9-loci ST), ST108 and ST109 (21-loci ST). The 2 STs were most genetically closed to the STs belonging to B. abortus. The results provide scientific basis for the control and prevention of brucellosis in Beijing.
吕燕宁,郭潇潇,陈丽娟,吕冰,常建华,华文浩,王慧珠,黎新宇,王全意. 两株布鲁菌MLST新序列型(ST)的鉴定[J]. 中国人兽共患病学报, 2018, 34(5): 434-440.
LYU Yan-ning, GUO Xiao-xiao, CHEN Li-juan, LYU Bing, CHANG Jian-hua, HUA Wen-hao, WANG Hui-zhu, LI Xin-yu, WANG Quan-yi. Identification of two new sequence type of Brucella isolates by multilocus sequence typing. Chinese Journal of Zoonoses, 2018, 34(5): 434-440.
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