Phylogenetic analysis based on small subunit ribosomal RNA (SSU rRNA) of Leishmania in Hancheng City, Shaanxi Province, China
ZHANG Zheng1, LI Xin-yan1,2, LIU Dong-li1, WANG Feng-ping3, WANG An-li1, WANG Tian-hai3
1. Shaanxi Provincial Center for Disease Control and Prevention, Xi’an 710054, China; 2. Health Science Center Xi’an Jiaotong University,Xi’an 710061, China; 3. Hancheng City Center for Disease Control and Prevention, Hancheng 715400, China
Abstract：We identified the species and molecular genetic characteristics of Leishmania protozoa in Hancheng City, Shaanxi Province. The SSU rRNA genes of Leishmania positive samples were amplified and sequenced. The sequencing results were compared with the SSU rRNA gene sequences of Leishmania in different regions and species reported in the literature. The mutation sites were analyzed and the phylogenetic tree was constructed. Three specimens’ SSU rRNA gene amplification sequences, which were highly homologous to L.donovani, L.Chagas and L.infantum, were successfully sequenced. The sequences had one base variation, comparing with the L. infantum in the UQ-II mutant region. Phylogenetic tree analysis showed that Leishmania from Shaanxi Province, Gansu Province and L. infantum strains from Xinjiang were gathered into one cluster. Epidemiological data and SSU rRNA sequence analysis showed that the pathogens of visceral leishmaniasis in Hancheng City, Shaanxi Province should be Leishmania infantum.
张铮,李欣妍,刘东立,王凤萍,王安礼,王天海. 陕西省韩城市利什曼原虫SSU rRNA基因系统发育研究[J]. 中国人兽共患病学报, 2018, 34(6): 550-554.
ZHANG Zheng, LI Xin-yan, LIU Dong-li, WANG Feng-ping, WANG An-li, WANG Tian-hai. Phylogenetic analysis based on small subunit ribosomal RNA (SSU rRNA) of Leishmania in Hancheng City, Shaanxi Province, China. Chinese Journal of Zoonoses, 2018, 34(6): 550-554.
2018, Vol. 34 
