Efficacy of tacrine on Echinococcus granulosus in vitro
LIU Cong-shan, YIN Jian-hai, YAO Jia-qing, XUE Jian, TAO Yi, ZHANG Hao-bing
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention;Key Laboratory of Parasite and Vector Biology, Ministry of Health;National Center for International Research on Tropical Diseases, Ministry of Science and Technology;WHO Collaborating Centre for Tropical Diseases, Shanghai 200025, China
Abstract:In order to study the effects of tacrine as the acetylcholinesterase inhibitor on Echinococcus granulosus in vitro, the E. granulosus protosoleces and germinal cells were isolated and cultured in vitro. The protoscoleces and germinal cells were incubated with tacrine at the concentration of 4, 8, 10, 20 and 40 μg/mL over 3 days respectively. Then the protoscoleces were stained with 0.1% methylene blue and the percentage of vital protoscoleces were calculated from the number of live and dead parasites. And the inhibition rate of germinal cells were determined based on CCK-8 kit. At the same time, the ultrastructure changes of parasites were observed through transmission and scanning electron microscopy. The results showed that after 3 days of exposure to 20 and 40 μg/mL of tacrine, the percentage of dead protoscoleces was 100%, and the slight swelling of the soma region and the outline of tegument dispersed became evident in the dead protoscoleces. In addition, the ultrastructural alterations was obvious including the presence of numerous vacuoles and lipid droplets in the tegument. The activity inhibition rate on germinal cells was about 100% at 40 μg/mL tacrine, the disappearance of adhesion matrix, the aggregation and detaching from the culture plate of E. granulosus germinal cells had be seen as well. SEM revealed the contraction and collapse of germinal cells after tacrine treatment. It suggests that tacrine could directly effect the activity of E. granulosus protocoleces and germinal cells in vitro. Therefore, tacrine is a potential drug in the treatment of hydatid diseases.
刘丛珊, 尹建海, 姚嘉青, 薛剑, 陶奕, 张皓冰. 他克林体外抗细粒棘球蚴作用研究[J]. 中国人兽共患病学报, 2018, 34(7): 608-612.
LIU Cong-shan, YIN Jian-hai, YAO Jia-qing, XUE Jian, TAO Yi, ZHANG Hao-bing. Efficacy of tacrine on Echinococcus granulosus in vitro. Chinese Journal of Zoonoses, 2018, 34(7): 608-612.
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