Detection of 14 potential virulence genes on food-borne Listeria monocytogenes
QIAN Ling-xiao1, KANG Li-chao2, LI Hong-huan1, ZHANG Qi-wen1, DU Dong-dong1, MA Xun1
1. School of Animal Science and Technology, Shihezi University, Shihezi 832000, China; 2. Food Testing Center of Xinjiang Academy of Agricultural Reclamation, Shihezi 832000, China
Abstract:We investigated the carrying rate of potential virulence genes of foodborne Listeria monocytogenes (LM) in Xinjiang. Specific primers were designed and synthesized for 14 potential virulence genes, and these genes were detected by PCR from 54 food-borne LM strains isolated from the different foods. Results showed that the 54 isolates carried potential virulence genes. The 5 563 isolate had the highest carrying rate, which was 57.14%, the 21 L662 isolate had the lowest carrying rate, which was 21.43%. The carrying rate in other isolates ranged from 28.57%(4/14) to 50%(7/14). The detection rate of virulence genes of Oligopeptide transporter, Internalin-like protein, cell wall surface anchor family protein, monovalent cation/H+ antiporter, peptidoglycan-bound protein, histidine metabolism, synthetic lethal KAR3-like protein, arginine/ornithine antiporter, ABC transporter, typeⅡ restriction modification system Sau3 Al, PTS system celloblose specific genes synthesis of aromatic amino acids, were 98.15%, 81.48%, 77.78%, 64.81%, 59.26%, 53.70%, 51.85%, 35.19%, 12.96%, 9.26% and 9.26%, respectively. Synthesis of aromatic amino acids,epimerase/dehydratase family protein and transcriptional regulator, EsaC protein analog (EsaC) genes were not detected. Understanding the distribution of 14 potential virulence genes in the food-borne LM isolates laid the foundation for the study of the pathogenicity and the traceability of food LM.
钱凌霄, 康立超, 李红欢, 张奇文, 杜冬冬, 马勋. 食源性单增李斯特菌14种潜在毒力基因的检测[J]. 中国人兽共患病学报, 2018, 34(10): 878-882.
QIAN Ling-xiao, KANG Li-chao, LI Hong-huan, ZHANG Qi-wen, DU Dong-dong, MA Xun. Detection of 14 potential virulence genes on food-borne Listeria monocytogenes. Chinese Journal of Zoonoses, 2018, 34(10): 878-882.