Identification and biological characterization of a Macaca-source Campylobacter jejuni strain
JIN Wei-wei1,2,TANG Hai-yan1,2,REN Fang-zhe1,2,TANG Hong1,2,JIAO Xin-an1,2,HUANG Jin-lin1,2
1. Key Laboratory of Zoonoses of Jiangsu Province, Yangzhou University, Yangzhou 225009, China; 2. Co-Innovation Center for Prevention and Control of Important Animal Infection Diseases and Zoonoses of Jiangsu Province, Yangzhou University, Yangzhou 225009, China
Abstract:Campylobacter jejuni (C. jejuni) is one of the most common foodborne pathogens causing diarrhea, especially in the developed countries. However, the pathogenic mechanism of C. jejuni remains uncertain by now. The variation of the sequenced type strain NCTC1168 seems to be universal, bothering researchers to some extent. A strain named as M166, which was isolated from the anal swabs of a diarrhea Macaca with suspected C. jejuni infection, was confirmed as C. jejuni by multiple PCR, VITEK2 compact microbial identification system test, sodium hippurate hydrolysis experiment, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) test and electron microscope observation. Compared with NCTC11168, though the growth rate and proliferation of M166 in MH were not as good as that of NCTC11168, the motility of M166 was much more active than NCTC11168. M166-inoculated Balb/c mice carried C.jejuni in their feces constantly, and the loads of C.jejuni were detected in their duodenum, jejunum, ileum and cecum with 3.09±0.47, 3.01±0.84, 3.80±0.46, 5.63±0.34, respectively, while NCTC11168 wasn’t detected 7 days post-inoculation, which indicates that M166 has better colonization ability in intestinal tracts. Our study has observed that strain M166 is able to actively colonize in mice, which indicates its pathogenicity in Macaca, and a further research is required to evaluate the pathogenicity of this strain.
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