Abstract：The aim of this study is to breed and identify IFN-γ knockout mice and to investigate the infection of TgCatCHn4 Toxoplasma gondii strain in IFN-γ knockout mice. Frozen embryos of IFN-γ-/- mice were purchased from Jackson Laboratories, USA, and recovered by the Peking Union Medical College Hospital. IFN-γ+/- mice were bred, the ratio of female and male was 3:1. The tail tissues of the mice was collected and DNA was extracted. The expression of IFN-γ gene was identified by PCR. IFN-γ-/- mice and BALB/c mice were injected with TgCatCHn4 T. gondii subcutaneously, and the infection rate and survival of the mice were analyzed. The tachyzoites or number of cysts of mice were examined. T. gondii positive mice were inoculated into Vero cells to observe the growth of T. gondii. Results showed that the breeding and genetic identification of IFN-γ knockout mice were successful. After infection with TgCatCHn4 T. gondii, the survival time of IFN-γ-/- mice was 17.0±1.0 DPI, and a large number of T. gondii tachyzoites were observed in the lung. The survival time of BALB/c mice was 304.0±43.7 DPI, and the number of cysts was 2.6±1.7/mouse brain. It is indicated that IFN-γ-/- mice have a short survival time after infected with T. gondii, which was ideal animal model of acute toxoplasmosis.
苏瑞景, 董辉, 朱瑶, 杨玉荣. IFN-γ基因敲除小鼠的繁育及其对TgCatCHn4弓形虫的感染研究[J]. 中国人兽共患病学报, 2019, 35(3): 250-253.
SU Rui-jing, DONG Hui, ZHU Yao, YANG Yu-rong. Reproduction of IFN-γ knockout mice and infection of TgCatCHn4 strain of Toxoplasma gondii . Chinese Journal of Zoonoses, 2019, 35(3): 250-253.
[1] Secombes CJ, Zou J. Evolution of Interferons and Interferon receptors [J]. Front Immunol,2017, 8: 209. [2] Schroder K, Hertzog PJ, Ravasi T, et al. Interferon-gamma: an overview of signals, mechanisms and functions [J]. J Leukoc Biol, 2004, 75(2): 163-189. [3] 安春丽, 王继春, 王雪莲. IFN-γ 和IL-4抗卡氏肺孢子虫感染作用的研究[J]. 中国寄生虫学与寄生虫病杂志,2002, 5: 285-288. [4] Dubey JP. Toxoplasmosis of animals and humans [M]: CRC Press,2010: 1-313. [5] Dubey JP. The history of Toxoplasma gondii-the first 100 years [J]. J Eukaryot Microbiol,2008, 55 (6): 467-475. [6] Lehmann T, Marcet PL, Graham DH, et al. Globalization and the population structure of Toxoplasma gondii [J]. Proc Nati Acad Sci USA,2006, 103 (30): 11423-11428. [7] Howe DK, Sibley LD. Toxoplasma gondii comprises three clonal lineages: correlation of parasite genotype with human disease [J]. J Infect Dis,1995, 172 (6): 1561-1566. [8] Cheng W, Wang C, Xu T, et al. Genotyping of polymorphic effectors of Toxoplasma gondii isolates from China[J]. Parasit Vectors, 2017, 10(1): 580. [9] Yang YR, Feng YJ, Lu YY, et al. Antibody detection, isolation, genotyping, and virulence of Toxoplasma gondii in captive felids from China [J]. Front Microbiol,2017, 8: 1414. [10] Dubey JP, Ferreira LR, Martins J, et al. Oral oocyst-induced mouse model of toxoplasmosis: effect of infection with Toxoplasma gondii strains of different genotypes, dose, and mouse strains (transgenic, out-bred, in-bred) on pathogenesis and mortality [J]. Parasitology,2012, 139 (1): 1-13. [11] Jones TC, Bienz KA, Erb P. In vitro cultivation of Toxoplasma gondii cysts in astrocytes in the presence of gamma interferon[J]. Infect Immun, 1986, 51(1):147-156. [12] Sa Q, Ochiai E, Tiwari A, et al. Cutting edge: IFN-γ produced by brain-resident cells is crucial to control cerebral infection with Toxoplasma gondii[J]. J Immunol, 2015, 195(3): 796-800. [13] Yano A, Mun HS, Chin M, et al. Roles of IFN-gamma on stage conversion of an obligate intracellular protozoan parasite, Toxoplasma gondii[J]. Int Rev Immunol, 2002, 21(4/5):405-421. [14] Yang YR, Ying YQ, Verma SK, et al. Isolation and genetic characterization of viable Toxoplasma gondii from tissues and feces of cats from the central region of China [J]. Vet Parasitol, 2015, 211 (3/4): 283-288.
2019, Vol. 35 
