Isolation and identification of pathogenetic bacteria including Vibrio cholerae and Salmonella from wild birds
ZHU Ling-wei1, JING Jie1, LIANG Bing1, WANG Ying1, TIAN Yuan1, JI Xue1, SUN Yang1, CHU Dong2, GAO Yu-wei1, GUO Xue-jun1
1. Institute of Military Veterinary Medicine, AMMS, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, China; 2. State Forestry Administration, Shenyang 110000, China
Abstract:The pathogenetic bacteria were isolated from wild water birds following sudden death in Inner mongolia autonomous region of China, under both aerobic and anaerobic conditions. Those strains were identified as Vibrio cholerae, Salmonella and Clostridium perfringens etc. by Phoenix100 automatic microorganism identification instrument or 16s rRNA sequencing. The mainly virulence genes, serotype or toxinotype of different strains were detected by specific PCR assays. All the C. perfringens isolates were determined as toxinotype A. All the V. cholerae isolates were determined as Non-O1/O139 sero-group, which contain only the hemolysin gene but not the cholera toxin and toxin-coregulated pili genes. Since those zoonotic bacteria can be isolated from wild migratory birds, it should deserve more attention for the biosafety and public health issues.
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