Evaluation and application of four nucleic acid detection methods for Listeria monocytogenes
JI Shun-shi, YE Chang-yun
State Key Laboratory for Infection Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Abstract:Polymerase chain reaction (PCR), real-time fluorescence polymerase chain reaction (Real-time PCR), loop-mediated isothermal amplification (LAMP) and multiple cross displacement amplification (MCDA) were used to detect Listeria monocytogenes in pork samples, then a rapid, sensitive and specific nucleic acid detection method was selected for purpose of reducing detection time and increasing detection rate. According to the references, four nucleic acid methods based on the specific gene lmo0733 of L. monocytogenes were used for detection of the pathogen in the artificially contaminated samples and the raw meat samples. The reaction rate, detection sensitivity and detection specificity of four methods were compared. Our data indicated that the four nucleic acid methods could accurately detect L. monocytogenes in pure nucleic acid, artificially contaminated samples and raw pork meat samples. The reaction rate (30 minutes per whole process) and sensitivity with limit of detection (LOD) of 10 fg of DNA template per reaction for MCDA method are better than that of the other three methods. In conclusion, MCDA assay could be used as a rapid, sensitive and efficient method for detection of L. monocytogenes in meat product.
纪顺师, 叶长芸. 四种单增李斯特菌常用核酸检测方法的评价与应用[J]. 中国人兽共患病学报, 2019, 35(12): 1085-1091.
JI Shun-shi, YE Chang-yun. Evaluation and application of four nucleic acid detection methods for Listeria monocytogenes. Chinese Journal of Zoonoses, 2019, 35(12): 1085-1091.
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