Abstract:To explore the difference between Korthof and EMJH medium in culture Leptospira and its application value in leptospirosis. The culture process of 15 Leptospira groups was observed the differences in morphology, number and self-coagulation in the two culture media, and detected antibody in MAT test on the serum of suspected leptospirosis patients and those with outbreak of epidemic leptospirosis. Leptospira grew and multiplied in large quantities on the second day after inoculation in EMJH medium while the peak of propagation was only reached after one week in Korthof medium. The sensitivity of MAT test also was higher in the EMJH medium than that of Korthof medium. The EMJH medium can be used for rapid separation of Leptospira, which is favorable for early determination of the prevalence of leptospirosis and laboratory studies.
潘敏楠, 徐国英. 钩端螺旋体EMJH培养基实验室应用[J]. 中国人兽共患病学报, 2020, 36(4): 317-319.
PAN Min-nan ,XU Guo-ying. Application value of EMJH medium of Leptospira in laboratory. Chinese Journal of Zoonoses, 2020, 36(4): 317-319.
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