Abstract:Rabies virus glycoprotein (RVGP) is the only antigen that is exposed to the surface of viral particles, being capable of inducing the host to produce neutralizing antibodies. And it is the only viral component present in all new rabies vaccines. This paper reviews the various methods for expression immunogenic recombinant rabies virus glycoprotein (rRVGP) using prokaryotic system, eukaryotic system and viral vector system through a large numbers of literatures readings and summaries. It also summarizes the similarity of rRVGP and natural rabies of viral glycoprotein (RVGP) in structure and immune response to the body, which provides a certain idea for the study of rabies genetic engineering vaccine.
张莹辉, 姚文生, 康凯, 薛麒, 李启红, 陈小云, 杜吉革, 朱真, 印春生. 狂犬病病毒糖蛋白重组表达及其基因工程疫苗研究进展[J]. 中国人兽共患病学报, 2020, 36(1): 65-69.
ZHANG Ying-hui, YAO Wen-sheng, KANG Kai, XUE Qi, LI Qi-hong, CHEN Xiao-yun, DU Ji-ge, ZHU Zhen, YIN Chun-sheng. Research progress of recombinant expression of rabies virus glycoprotein and its genetic engineering vaccine. Chinese Journal of Zoonoses, 2020, 36(1): 65-69.
[1] Blanchard T.Rabies and other Lyssavirus diseases[J]. Lancet (North American Edition), 2004, 363(9424):1906-1907.DOI:10.1016/S0140-6736(04)16367-8 [2] Crick J.Rabies Virus genome[M]. Rabie in the Tropics,1985.DOI:10.1007/978-3-642-70060-6_2 [3] Gupta P K,Sharma S, Walunj SS, et al.A DNA vaccine that encodes Rabies virus glycoprotein lacking transmemrane domain enhances antibody response but not protection[J]. Acta Virol, 2006, 50(2):87.DOI:10.1097/01.aids.0000198085.39831.91 [4] Ertl HCJ.Novel vaccines to human Rabies[J]. Plos Neglect Tropl D, 2009, 3(9):e515.DOI:10.1371/journal.pntd.0000515 [5] Fernando Bastida-González, Celaya-Trejo Y, José Correa-Basurto, et al.Predicted 3D model of the Rabies virus glycoprotein trimer[J]. Biomed Res Int, 2016, 2016:1-11.DOI:10.1155/2016/1674580 [6] Koraka P, Bosch BJ, Cox M, et al.A recombinant Rabies vaccine expressing the trimeric form of the glycoprotein confers enhanced immunogenicity and protection in outbred mice[J]. Vaccine, 2014, 32(36):4644-4650.DOI:10.1016/j.vaccine.2014.06.058 [7] Gaudin Y, Ruigrok RWH, Knossow M, et al.Low-pH conformational changes of Rabies virus glycoprotein and their role in membrane fusion[J]. J Virol, 1993, 67(3):1365-1372.DOI:10.1016/0166-0934(93)90022-J [8] Renata DFB, Hudacek A, Sawatsky B, et al.Inactivated recombinant Rabies viruses displaying canine distemper virus glycoproteins induce protective immunity against both pathogens[J]. J Virol, 2017, 91(8):e02077-16.DOI:10.1128/JVI.02077-16 [9] 杨正时,严子林.狂犬病疫苗的发展与现状及评述[J].中国微生态学杂志,2013,25(02):217-222.DOI:10.13381/j.cnki.cjm.2013.02.008 [10] Johnson N, Cunningham AF, Fooks AR.The immune response to Rabies virus infection and vaccination[J]. Vaccine, 2010, 28(23):3896-3901.DOI:10.1016/j.vaccine.2010.03.039 [11] Cruz E T, Iris Angélica Feria Romero, Juan Gabriel López Mendoza, et al. Efficient post-exposure prophylaxis against Rabies by applying a four-dose DNA vaccine intranasally[J]. Vaccine, 2008, 26(52):6936-6944.DOI:10.1016/j.vaccine.2008.09.083 [12] Ortiz-Prado E, Ponce J, Darío Ramírez, et al.Rabies epidemiology and control in ecuador[J]. Glob J Health Sci, 2016, 8(3):113-121.DOI:10.5539/gjhs.v8n3p113 [13] Omezuruike OI.Rabies vaccines: its role, challenges, considerations and implications for the global control and possible eradication of Rabies[J]. Int J Anim Vet Adv, 2010, 2(4). [14] Shakin-Eshleman S H, Remaley A T, Eshleman J R, et al. N-linked glycosylation of Rabies virus glycoprotein. Individual sequons differ in their glycosylation efficiencies and influence on cell surface expression[J]. J Biol Chem, 1992, 267(15):10690-10698.DOI:10.1016/0014-5793(92) 80497-5 [15] Maillard AP, Gaudin Y.Rabies virus glycoprotein can fold in two alternative, antigenically distinct conformations depending on membrane-anchor type[J]. J Gen Virol, 2002, 83(Pt 6):1465-1476.DOI:10.1099/0022-1317-83-6-1465 [16] Rath A, Choudhury S, Batra D, et al.DNA vaccine for Rabies: Relevance of the trans-membrane domain of the glycoprotein in generating an antibody response[J]. Virus Res, 2005, 113(2):143-152.DOI:10.1016/j.virusres.2005.05.002 [17] Qian W, Aguilar F, Wang T, et al.Secretion of truncated recombinant Rabies virus glycoprotein with preserved antigenic properties using a co-expression system in Hansenula polymorpha[J]. J Microbiol,2013,51(2):234-240.DOI:10.1007/s12275-013-2337-0 [18] Gaudin Y, Moreira S, Bénéjean J, et al. Soluble ectodomain of Rabies virus glycoprotein expressed in eukaryotic cells folds in a monomeric conformation that is antigenically distinct from the native state of the complete, membrane-anchored glycoprotein[J]. J Gen Virol,1999, 80 (Pt 7)(7):1647-1656. [19] Liwei Yan, Wei Gong, Wenbing Zhu, et al.Expression and purification of Rabies virus glycoprotein and analysis of its specific binding capacity to memory B cells[J].Chin J Biotechnol,Nov 25, 2017, 33(11): 1840-1849.DOI:10.13345/j.cjb.170060 [20] Pourcq K D, Schutter K D, Callewaert N.Engineering of glycosylation in yeast and other fungi: current state and perspectives[J].Appl Microbio Biot, 2010, 87(5):1617-1631.DOI:10.1007/s00253-010-2721-1 [21] Singh A, Saxena G, Verma P C.Oral Rabies vaccine design for expression in plants[J]. Methods MolBiol, 2016, 1404:547-567.DOI:10.1007/978-1-4939-3389-1_36 [22] Loza-Rubio E, Rojas-Anaya E, Juan López, et al.Induction of a protective immune response to Rabies virus in sheep after oral immunization with transgenic maize, expressing the Rabies virus glycoprotein[J]. Vaccine, 2012, 30(37):5551-5556.DOI:10.1016/j.vaccine.2012.06.039 [23] Wang XL, Cchen FF, Yuan W, et al.Expression of insect cell-based Rabies virus glycoprotein and assessment of its immunogenicity efficacy in mice[J]. J Nanjing Med University(Nat Sci), 2015.DOI:10.1128/CVI.05258-11 [24] 陈奇,张守峰,刘晔,等.狂犬病病毒糖蛋白的杆状病毒表达及其免疫原性研究[J].病毒学报,2012,28(5):501-505.DOI:10.13242/j.cnki.bingduxuebao.002311 [25] Moraes AM, Moraes, Jorge SAC, Astray R M, et al. Drosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development[J]. Biotechnol Adv, 2012, 30(3):613-628.DOI:10.1016/j.biotechadv.2011.10.009 [26] Yokomizo AY, Jorge SAC, Astray R M, et al.Rabies virus glycoprotein expression in Drosophila S2 cells. I. Functional recombinant protein in stable co-transfected cell line[J]. Biotechnol J, 2007, 2(1):102-109.DOI:10.1002/biot.200600211 [27] Butler M.Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals[J]. Appl Microbio Biot, 2005, 68(3):283-291.DOI:10.1007/s00253-005-1980-8 [28] Sakai M, Kankanamge P J, Shoji J, et al.Studies on the conditions required for structural and functional maturation of Rabies Virus glycoprotein (G) in G cDNA-transfected cells[J]. Microbiol Immunol, 2004, 48(11):853-864.DOI:10.1111/j.1348-0421.2004.tb03617.x [29] Jacobs B L, Langland J O, Kibler K V, et al.Vaccinia virus vaccines: Past, present and future[J]. Antiviral Res, 2009, 84(1):1-13.DOI:10.1016/j.antiviral.2009.06.006 [30] Maki J, Guiot A L, Aubert M, et al.Oral vaccination of wildlife using a vaccinia-Rabies-glycoprotein recombinant virus vaccine (RABORAL V-RG): a global review[J]. Vet Res, 2017, 48(1):57.DOI:10.1186/s13567-017-0459-9 [31] Fehlnergardiner C, Rudd R, Donovan D, et al.Comparing ONRAB(R) and RABORAL V-RG(R) oral Rabies vaccine field performance in raccoons and striped skunks, New Brunswick, Canada, and Maine, USA[J]. J Wildl Dis, 2012, 48(1):157-167.DOI:10.7589/0090-3558-48.1.157 [32] Brown LJ, Rosatte RC, Fehlnergardiner C, et al.Oral vaccination and protection of red foxes (Vulpes vulpes) against Rabies using ONRAB○R, an adenovirus-Rabies recombinant vaccine[J]. Vaccine, 2014, 32(8):984-989.DOI:10.1016/j.vaccine.2013.12.015 [33] 王颖,张守峰,刘晔,等.狂犬病病毒糖蛋白重组人腺病毒的构建及免疫效果的初步研究[J].病毒学报,2011,27(05):442-446.DOI:10.13242/j.cnki.bingduxuebao.002203 [34] Ge J, Wang X, Tao L, et al.Newcastle disease virus-vectored Rabies vaccine is safe, highly immunogenic, and provides long-lasting protection in dogs and cats[J]. J Virol, 2011, 85(16):8241-8252.DOI:10.1128/JVI.00519-11 [35] 于桂梅. 表达狂犬病毒G蛋白的重组新城疫病毒接种牛羊的免疫原性研究[D].北京:中国农业科学院,2015. DOI:10.7666/d.Y2787520.DOI10.7666/d.Y2787520 [36] 袁子国,张才,王晓虎,等.表达狂犬病病毒CVS-11株糖蛋白的重组伪狂犬病病毒在犬体内的免疫效果[J].中国兽医科学,2009,39(11):984-989. DOI:10.16656/j.issn.1673-4696.2009.11.010 [37] 李业伟. 表达狂犬病病毒糖蛋白重组伪狂犬病毒的构建及免疫原性研究[D].长春:吉林大学,2012. [38] Huang Y, Chen Z, Huang J, et al.Parainfluenza virus 5 expressing the G protein of Rabies virus protects mice after Rabies virus infection[J]. J Virol, 2015, 89(6):3427-3429.DOI:10.1128/JVI.03656-14 [39] 李翠霞. 表达狂犬病病毒G蛋白重组犬瘟热病毒Snyder Hill株的研究[D].北京:中国农业科学院,2018. [40] Ramya R, Mohana Subramanian B, Sivakumar V, et al.Expression and solubilization of insect Cell-Based Rabies Virus glycoprotein and assessment of its immunogenicity and protective efficacy in Mice[J]. Clin Vaccine Immunol, 2011, 18(10):1673-1679.DOI:10.1128/CVI.05258-11