Abstract：The aim of this study was to investigate the prevalence of canine leptospirosis in Beijing. Blood samples and urine samples (n=1 600) were collected from pet hospitals, kennels and shelters during January 2017 to February 2019. All samples were performed by quantitative real-time polymerase chain reaction(qPCR) assays. Then antibody detection and conventional PCR assays were conducted for qPCR-positive samples. The overall positive rate of qPCR was 1.01% (17/1 600). While the positive rate of urine samples (2.00%) was significant higher than that of blood samples (0.13%). The chi-square test showed region(χ2=17.828, P<0.05) season (χ2=10.916, P<0.05) and source(χ2=9.432, P<0.01) were major risk factors associated with leptospirosis. However, no significant differences were found among age (χ2=1.103, P=0.613), gender (χ2=1.124, P=0.223) and breed (χ2=0.427, P=0.448) in infected dogs. Microscopic agglutination test (MAT) results showed 12 serum samples were positive for the antibodies of Australia serogroup and Ballum serogroup. The isolates were identified as Leptospira interrogans by PCR . This study indicates the canine leptospirosis prevalence in part of Beijing. Disease surveillance and vaccination at a regular interval should be recommended for dog owners to reduce the risk of infection and transmission to human.
[1] Adler B.Leptospira and leptospirosis[M]. Missouri: Elsevier Inc, 2015. DOI: 10.1007/978-3-662-45059-8 [2] Zhang C, Wang H, Yan J.Leptospirosis prevalence in Chinese populations in the last two decades[J]. Microbes & Infection, 2012, 14(4):317-323. DOI: 10.1016/j.micinf.2011.11.007 [3] 严杰, 戴保民, 于恩庶. 钩端螺旋体病学[M].北京:人民卫生出版社, 2006. [4] Sykes JE, Hartmann K, Lunn KF, et al.2010 ACVIM small animal consensus statement on leptospirosis: diagnosis, epidemiology, treatment, and prevention[J]. J Vet Intern Med, 2011, 25(1):13. DOI: 10.1111/j.1939-1676.2010.0654.x [5] Gravekamp C, Kemp HVD, Franzen M, et al.Detection of seven species of pathogenic leptospires by PCR using two sets of primers[J]. J Gen Microbiol, 1993, 139(8):1691-1700.DOI: 10.1099/00221287-139-8-1691 [6] Ahmed N, Devi S M, Valverde MDLA, et al.Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species[J]. Ann Clin Microb Anti, 2006, 5(5):28. DOI: 10.1186/1476-0711-5-28 [7] 张宇翔, 聂一新, 李秀文,等. 钩端螺旋体病MAT检测方法的标准化及结果准确性国际评测[J]. 疾病监测, 2006, 21(5):229-232. DOI: 10.3784/j.issn.1003-9961.2006.5.229 [8] Hu W, Lin X, Yan J.Leptospira and leptospirosis in China[J]. Curr Opin Infect Dis, 2014, 27(5):432-436. DOI: 10.1097/QCO.0000000000000097 [9] 秦进才, 马颖, 王岳竹, 等. 在北京地区调查发现拜伦型钩端螺旋体病[J]. 中国人兽共患病学报, 1992, 8(05):35-36. [10] Glickman LT, Moore GE, Glickman NW, et al.Purdue university-banfield national companion animal surveillance program for emerging and zoonotic diseases[J]. Vector-Borne Zoonotic Dis, 2006, 6(1):14-23. DOI: 10.1089/vbz.2006.6.14 [11] Major A, Schweighauser A, Francey T.Increasing incidence of canine leptospirosis in switzerland[J]. Inter J Env Res Pub Heal, 2014, 11(7):7242-7260. DOI: 10.3390/ijerph110707242 [12] 齐海霞, 张海云, 邓小雨,等. 犬、猫钩端螺旋体病的流行病学调查[J]. 中国畜牧兽医, 2012, 39(11):203-206. DOI: 10.3969/j.issn.1671-7236.2012.11.047 [13] Fraune CK, Schweighauser A, Francey T.Evaluation of the diagnostic value of serologic microagglutination testing and a polymerase chain reaction assay for diagnosis of acute leptospirosis in dogs in a referral center[J]. J Am Vet Med Assoc, 2013, 242(10): 1373-1380. DOI: 10.2460/javma.242.10.1373 [14] Greene CE.Infectious diseases of the dog and cat, 4th ed[M]. St. Louis, Missouri: Elsevier Inc, 2012. DOI: 10.1111/j.1751-0813.1999.tb11241.x [15] Ward MP, Glickman LT, Guptill L F.Prevalence of and risk factors for leptospirosis among dogs in the United States and Canada: 677 cases (1970-1998)[J]. J Am Vet Med Assoc, 2002, 220(1):53-58. DOI: 10.2460/javma.2002.220.53 [16] Koizumi N, Muto MM, Akachi S, et al.Molecular and serological investigation of Leptospira and leptospirosis in dogs in Japan[J]. J Med Microbiol 2013, 62(Pt_4):630-636. [17] Lee HS, Levine M, Guptill-Yoran C, et al.Regional and temporal variations of Leptospira seropositivity in dogs in the United States, 2000-2010[J]. J Vet Intern Med, 2014, 28(3): 779-788. DOI: 10.1111/jvim.12335
2020, Vol. 36 
