Pathogenicity and protection in mice of rearranging the M gene of RABV HEP-Flury
MEI Ming-zhu1,2, YANG Xian-feng2, LONG Teng2, ZHANG Qiong2, ZHAO Jing2, TIAN Qin2, PENG Jiao-jiao2, LUO Jun2, JIANG He2, LIN Ying-yi2, LIN Zhi-xiong1, GUO Xiao-feng2
1.Guangzhou Custom Technology Center, Guangzhou 510623, China; 2.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
Abstract：In order to help rabies vaccine candidate construction, the lethality and immunological protection between rHEP-Flury and M gene rearranged viruses were compared and analyzed. Lethality in suckling mice was performed by intracranial inoculating rHEP-Flury or M gene rearranged viruses, then LD50 was calculated. Protection of mice was performed by immunized once intramuscularly with different doses of rHEP-Flury or M gene rearranged viruses, then mice were challenged IC with CVS-24 and survivor numbers were evaluated. At the same time, the antibody level was assessed by ELISA. The lethality in suckling mice increased as the M gene was moved successively away from promoter proximal position to successive positions down the viral genome, that is M2<rHEP-Flury<M4. Protection of mice showed that the percent survival at the dose of 104 and 105FFU has no significant difference, however, at the dose of 103FFU, the percent survival of M gene rearranged viruses was clearly higher than that of rHEP-Flury. M gene rearrangement of HEP-Flury could decrease the viral lethality and increase the protection, which is benefit for the choose of rabies vaccine candidate.
梅明珠,杨先锋,龙腾,张琼,赵静,田钦,彭娇娇,罗均,姜贺,林颖仪,林志雄,郭霄峰. 狂犬病病毒HEP-Flury M基因重排在小鼠致病性和保护性研究[J]. 中国人兽共患病学报, 2020, 36(3): 173-177.
MEI Ming-zhu, YANG Xian-feng, LONG Teng, ZHANG Qiong, ZHAO Jing, TIAN Qin, PENG Jiao-jiao, LUO Jun, JIANG He, LIN Ying-yi, LIN Zhi-xiong, GUO Xiao-feng. Pathogenicity and protection in mice of rearranging the M gene of RABV HEP-Flury. Chinese Journal of Zoonoses, 2020, 36(3): 173-177.
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2020, Vol. 36 
