Molecular characterization analysis of Brucella strains isolated from human in Jiangsu Province, 2018
TAN Zhong-ming1, WANG Xiu-bin2, ZHOU Wei-zhong1, DONG Chen1, ZHOU Lu1, HONG Jie1, QIAN Hui-min1, HU Jian-li1, BAO Chang-jun1
1.NHC Key laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009,China; 2.Hangzhou Xiaoshan Yinong Town Community Health Service Center, Hangzhou 311200,China
Abstract:Species types and genotypes of the major human Brucella strains in Jiangsu province in 2018 were determined in this study. The genotypes of the isolates were determined by AMOS multiple PCR. The multi-locus sequence analysis (MLSA) and multiple-locus variable number tandem repeat analysis (MLVA) were used to identify genotypes, and cluster analysis was performed to differentiate domestic and foreign popular strains. A total of 63 strains were isolated in Jiangsu, 2018. The MLSA showed that one strain was ST17, and the others were ST8. 56 strains were divided into 47 gene subtypes by the MLVA (There were forty-six genotypes of B. melitensises and one of B. suis), and cluster analysis showed that all the Brucella melitensis strains were clustered in the "East Mediterranean clusters group". The results showed that the predominant species of Brucella in 2018 were ST8 B. melitensis. Moreover, human infected with ST17 Brucella suis strain was first report in Jiangsu Province.
谈忠鸣, 汪秀斌, 周伟忠, 董晨, 周璐, 洪捷, 钱慧敏, 胡建利, 鲍倡俊. 2018年江苏省人感染布鲁氏菌分离株分子特征分析[J]. 中国人兽共患病学报, 2020, 36(7): 555-560.
TAN Zhong-ming, WANG Xiu-bin, ZHOU Wei-zhong, DONG Chen, ZHOU Lu, HONG Jie, QIAN Hui-min, HU Jian-li, BAO Chang-jun. Molecular characterization analysis of Brucella strains isolated from human in Jiangsu Province, 2018. Chinese Journal of Zoonoses, 2020, 36(7): 555-560.
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