Analysis of predominant enterovirus and serotypes causing respiratory infection in children and their epidemiological features
WANG Min1, 2, YANG Ai-ping2, CHEN Yan2, HU Ping-fang2, YAN Jie1
1. Department of Pathogen Biology and Microbiology, Zhejiang University School of Medicine, Hangzhou 310058, China; 2. Zhejiang Xiaoshan Hospital, Hangzhou 311200, China
Abstract:To investigate the predominant enterovirus (EVs) and their serotypes causing respiratory infection in children as well as the epidemic features and susceptible population of the pathogens. The throat swab samples from 2 164 children with fever and acute respiratory infection symptoms in the local area during 2016 to 2018 were collected. The nucleic acids of EVs in the samples were detected by real-time fluorescent quantitative RT-PCR using EVs-universal primers. The viral nucleic acid-positive samples were amplified by Nested-PCR using EV-VP1/VP2 gene-universal primers for sequencing and typing. The epidemiological features such as sex, age and infected predominant EVs and their serotypes of the children were analyzed. The results showed that 508 cases (23.5%) of the 2 164 throat swab samples were positive for nucleic acids of EVs, in which 290 cases were successfully sequenced for typing. In the detected EVs, coxsackievirus group A (CoxA) and group B (CoxB) isolates were accounting for 75.2% and 11.7% while EV71 and echovirus isolates were made up to 8.9% and 4.1%, respectively. The positive rate of CoxA was significantly higher than that of CoxB, EV71 and echovirus (P<0.05). In the CoxA-positive samples, the detection rates of CoxA6 (39.5%) and CoxA10 (3.0%) were significantly higher than that of CoxA16, CoxA4 and CoxA2 (P<0.05). The 1-3 aged male children were the susceptible population (P<0.05), in which the male and female children were susceptible for CoxA6 and CoxA10, respectively (P<0.05). All the data indicated that EVs are the important pathogens of respiratory infection in children in the local area, and coxsackievirus and its CoxA6 and CoxA10 are the predominant pathogen and its serotypes in the EVs as well as 1-3 aged children were the major susceptible population for the infection in the local area but the male children are more susceptible than the female children.
汪敏, 杨爱平, 陈燕, 胡萍芳, 严杰. 引起儿童呼吸道感染的优势肠道病毒及其血清型和流行特征分析[J]. 中国人兽共患病学报, 2020, 36(6): 469-474.
WANG Min, YANG Ai-ping, CHEN Yan, HU Ping-fang, YAN Jie. Analysis of predominant enterovirus and serotypes causing respiratory infection in children and their epidemiological features. Chinese Journal of Zoonoses, 2020, 36(6): 469-474.
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