Abstract：To study the mechanism of antimicrobial resistance and the molecular characteristic of ESBL-producing E.coli isolates identified from rodents. During September to November 2016, 123 rodents were captured from one urban district in Guangzhou, including 57 Rattus flavipectus, 54 Rattus norvegicus, 11 Suncus murinus and 1 Rattus rattus. Thirty-five cefotaxime resistant E.coli isolates were identified as ESBL-producing strains in intestine samples of the rodents. Antimicrobial susceptibility of the isolates determined by vitek2 compact showed that the resistance rate to ampicillin, ampicillin/sulbactam, piperacillin, piperacillin/tazobactam, cefotaxime, ceftazidime, ceftriaxone, cefepime, levofloxacin and ciprofloxacin were 100%(35/35)、91.4%(32/35)、97.1%(34/35)、100%(35/35)、100%(35/35)、88.6%(31/35)、80%(28/35) and 74.3%(26/35), respectively. Thirty-seven CTX-M genes were detected in the 35 isolates. The most predominant CTX-M-type genes were blactx-m-79 (n=23), followed by blactx-m-55 (n=6),blactx-m-123 (n=4),blactx-m-15 (n=2),blactx-m-65 (n=1) and blactx-m-27 (n=1). Molecular typing of the 35 isolates was performed by multilocus sequence typing (MLST) and pulsed field gel electrophoresis (PFGE). The results indicated the CTX-M producing isolates were genetically diversified. In conclusion, our results showed high prevalence of ESBL-producing E.coli from rodents in Guangzhou. Carrying CTX-M type genes is the mechanism of the isolates resistant to cefotaxime. The CTX-M type genes detected in the present study were also found in healthy people and hospitalized patients from previous studies. The MLST and PFGE results found that the CTX-M producing isolates were genetically diversified. This study indicated rodent-like animals can be the reservoir host of ESBL-producing E.coli.
徐建敏, 侯水平, 张晶, 陶霞, 白志军, 吴新伟, 周勇. 广州市鼠形动物肠道产ESBL大肠埃希菌分布及病原特征分析[J]. 中国人兽共患病学报, 2020, 36(7): 561-565.
XU Jian-min, HOU Shui-ping, ZHANG Jing, TAO Xia, BAI Zhi-jun, WU Xin-wei, ZHOU Yong. Study on the antimicrobial resistance mechanism of ESBL-producing Escherichia coli isolated from rodents in Guangzhou. Chinese Journal of Zoonoses, 2020, 36(7): 561-565.
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2020, Vol. 36 
