Identification and virulence gene analysis of the first human isolate of Streptococcus suis type 14 in Hunan Province,China
HE Zi-xiang1, XIA Xin1, QIN Yu-fang3, YANG Hao1, QIN Di1, XIANG Xing-yu1, HU Jun-zhong2, ZHAN Zhi-fei1
1. Hunan Provincial Center for Disease Control and Prevention,Changsha 410005,China; 2. Zhangjiajie Center for Disease Control and Prevention,Zhangjiajie 427000,China; 3. Yongding district Center for Disease Control and Prevention Zhangjiajie 427000,China
Abstract：In order to investigate the biological characters and pathogenic characteristics of the first strain of Streptococcus suis type 14 in Hubei Province. The patient’s joint fluid was collected and inoculated into blood agar plate for isolation and culture of Streptococcus suis. The strain was confirmed by microscopic examination, biochemical and serum agglutination test. Polymerase chain reaction (PCR) was used to detect Streptococcus suis species-specific gene (16S rRNA), type 2 capsular polysaccharide gene (cps2J), type 14 capsular polysaccharide gene (cps14J) and related virulence genes: lysozyme release related protein (mrp), hemolysin (sly), fibronectin binding protein (fbps), extracellular factor (ef), glutamate dehydrogenase (gdh), the virulence-related sequence of orf2 (orf2) and the coding gene for glyoxyl-monophosphate dehydrogenase (gapdh). The results showed that the Streptococcus suis type 14 strain was isolated from the joint fluid of the patient. The species-specific gene 16S rRNA was positive. Capsular polysaccharide gene: cps2J was negative, while cps14J was positive. The virulence gene: mrp was negative, and the remaining sly, fbps, ef, gdh, orf2, gapdh were positive. In conclusion,the prevalence of Streptococcus suis type 14 carrying a variety of virulence genes has appeared in Hunan Province, and it is necessary to pay close attention to its prevalence trends.
贺子翔, 夏昕, 覃玉芳, 杨浩, 覃迪, 向星宇, 胡俊忠, 湛志飞. 湖南省首例猪链球菌14型人源分离株鉴定及毒力基因分析[J]. 中国人兽共患病学报, 2020, 36(8): 631-635.
HE Zi-xiang, XIA Xin, QIN Yu-fang, YANG Hao, QIN Di, XIANG Xing-yu, HU Jun-zhong, ZHAN Zhi-fei. Identification and virulence gene analysis of the first human isolate of Streptococcus suis type 14 in Hunan Province,China. Chinese Journal of Zoonoses, 2020, 36(8): 631-635.
[1] 杨绍基,任红.传染病学[M].7版.北京:人民卫生出版社,2011:206-210. [2] Heidt MC, Mohamed W, Hain T, et al.Human infective endocarditis caused by Streptococcus suis serotype 2[J]. J Clin Microbiol, 2005, 43(9):4898-4901. DOI:10.1128/JCM.43.9.4898-4901.2005 [3] Leelarasamee A, Nilakul C, Tien-Grim S, et al.Streptococcus suis Toxic-shock Syndrome and Meningitis[J]. J Med Assoc Thai, 1997, 80(1):63-68. [4] Dominguez-Punaro MC, Segura M, Plante MM, et al.Streptococcus suis Serotype 2, an important swine and human pathogen, induces strong systemic and cerebral inflammatory responses in a mouse model of infection[J]. J Immunol, 2007, 179(3):1842-1854. DOI:10.4049/jimmunol.179.3.1842 [5] 吕燕宁,李洁,杜轶威,等. 一株人血源猪链球菌的分离鉴定与毒力基因检测[J]. 中国人兽共患病学报,2017, 33(7):599-603. DOI:10.3969/j.issn.1002-2694.2017.07.005 [6] 李小军. 上海地区致病性猪链球菌及其毒力基因的检测[D]. 南京:南京农业大学, 2007. [7] 湛志飞,张红,胡世雄,等. 湖南省首例人感染猪链球菌病的病原分离与鉴定[J]. 中国热带医学,2007,7(5):702-703. [8] Zhijie L, Han Z, Marcelo G, et al.Development of multiplex PCR assays for the identification of the 33 serotypes of Streptococcus suis[J]. PLoS One, 2013, 8(8):e72070. DOI:10.1371/journal.pone.0072070 [9] Tan C,Liu ML,Jin ML,et al.The key virulence-associated genes of Streptococcus suis type 2 are upregulated and differentially expressed in vivo[J]. FEMS Microbiology Letters, 2008, 278(1):108-114. DOI:10.1111/j.1574-6968.2007.00980.X [10] 方勤美,黄绍谦,俞伏松,等. 猪链球菌2型福建分离株的主要毒力基因分析[J]. 中国人兽共患病学报,2010,26(1):65-68. DOI:10.3969/j.issn.1002-2694.2010.01.016 [11] 尹建雯,徐闻,古文鹏,等. 云南省首例人感染猪链球菌病的病原学分析[J]. 应用预防医学,2012, 18(2):78-80. [12] 董德平,韩立中,项明洁,等. 猪链球菌Ⅱ型致败血症一例[J]. 中华医学检验杂志,1999,22(2):73. DOI:10.3760/j.issn.1009-9158.1999.02.022 [13] 祝小平,祖荣强,陈志海,等. 四川省人感染猪链球菌病死亡病例特征分析[J]. 中华流行病学杂志,2005,26(9):633-635. [14] 马丽梅,谭丽娟. VITEK2 compact微生物分析系统对猪链球菌2型快速鉴定[J]. 检验医学与临床,2012, 9(13):1617-1619. [15] 江南,杨兴祥,唐荣珍,等. 四川省确诊人猪链球菌病48例临床特征[J]. 中华传染病杂志,2006,24(3):179-182. DOI:10.3760/j.issn.1000-6680.2006.03.011 [16] 许晓燕. 猪链球菌对四环素耐药机制的研究及耐药基因三重PCR检测方法的建立[D].泰安:山东农业大学,2011. DOI:10.7666/d.d144176 [17] 刘荻萩,李艳华,蔡雪辉,等. 链球菌对抗生素产生耐药性的作用机制[J]. 国外医药(抗生素分册),2007,(3):103-107. DOI:10.3969/j.issn.1001-8751.2007.03.002 [18] 孙珂,倪艳秀,祝昊丹,等. 猪链球菌4型分离株生物学特性研究[J]. 中国畜牧兽医,2019, 46(1):80-88. DOI:10.1643/j.cnki.1671-7236.2019.01.010 [19] 张振江,杜毅超,任鹏飞,等. 猪9型链球菌的分离鉴定及其生物学特性研究[J]. 动物医学进展,2018, 39(4):36-41. [20] 王淑杰,段贵鑫,刘永刚,等. 一株血清31型猪链球菌的分离鉴定及生物学特性研究[J]. 中国预防兽医学报,2018, 40(4):347-349. DOI:10.3969/j.issn.1008-0589.201705005 [21] Smith HE, Rijnsburger M, Stockhofe-Zurwieden N, et al.Virulent strains of Streptococcus suis serotype 2 and highly virulent strains of Streptococcus suis serotype 1 can be recognized by a unique ribotype profile[J]. J Clin Microbiol, 1997, 35(5):1049-1053. DOI:10.1128/JCM.35.5.1049-1053.1997
2020, Vol. 36 
