细粒棘球蚴和多房棘球蚴感染对Balb/c小鼠淋巴细胞亚群的影响

doi:10.3969/j.issn.1002-2694.2021.00.057

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摘要目的分析Balb/c小鼠感染细粒棘球蚴和多房棘球蚴后脾脏和血液淋巴细胞亚群的变化。方法利用流式细胞仪分析感染细粒棘球蚴和多房棘球蚴的Balb/c小鼠脾脏和血液中T、B、Th1、Th2、Treg、NK细胞亚群变化。结果脾脏B、Th1、Th2、Treg细胞在多房棘球蚴感染组和对照组比较,差异有统计学意义;血液Th1和Th2细胞在多房棘球蚴感染组和对照组比较,差异有统计学意义(t_Th1=3.669, P<0.01; t_Th2=4.302, P<0.01)。脾脏B、Th1、Th2、Treg细胞在细粒棘球蚴感染组和对照组比较,差异有统计学意义(t_B=3.396,P<0.05;t_Th1=3.539,P<0.05;t_Th2=3.731,P<0.05;t_Treg=2.197,P<0.05);血液B、NK、Th1、Th2细胞在细粒棘球蚴感染组和对照组之间比较,差异有统计学意义(t_B=2.125,P<0.05;$t_{N_{K}}$=3.569,P<0.05;t_Th1=6.532,P<0.05;t_Th2=4.463,P<0.05)。结论小鼠脾脏B、Th1、Th2、Treg细胞在细粒棘球蚴和多房棘球蚴感染组变化趋势基本一致。多房棘球蚴感染组血液中Th1和Th2细胞有变化,细粒棘球蚴感染组血液中B、NK、Th1和Th2细胞有变化。

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	曹得萍
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	陈根

关键词 ：细粒棘球蚴, 多房棘球蚴, Balb/c小鼠, 脾脏, 血液, 淋巴细胞亚群

Abstract：This study aimed to analyze lymphocyte subsets in spleen and blood of Balb/c mice infected by hydatid cyst and alveolar hydatid. Flow cytometry was used to analyze T, B, Th1, Th2, Treg and NK lymphocyte in spleen and blood of Balb/c mice with hydatid cyst and alveolar hydatid infection. B, Th1, Th2 and Treg cells in spleen of mice with alveolar hydatid infected group and control group were statistical difference; The difference of Th1 and Th2 cells between the alveolar hydatid infected group and the control group were statistical significance (t_Th1=3.669, P<0.01; t_Th2=4.302, P<0.01). B, Th1, Th2 and Treg cells in spleen of mice with hydatid cyst infected group and control group were statistical difference (t_B=3.396,P<0.05;t_Th1=3.539,P<0.05;t_Th2=3.731,P<0.05;t_Treg=2.197,P<0.05); B, NK, Th1 and Th2 cells between the hydatid cystgroup and the control group were statistical significance (t_B=2.125,P<0.05;$t_{N_{K}}$=3.569,P<0.05;t_Th1=6.532,P<0.05;t_Th2=4.463,P<0.05). B, Th1, Th2 and Treg cells in both hydatid cyst and alveolar hydatid infected group showed the same trend. There were changes of Th1 and Th2 cells in the blood of alveolar hydatid infected group. There were changes of B, NK, Th1 and Th2 in the blood of hydatid cyst infected group.

Key words： hydatid cyst alveolar hydatid Balb/c mice spleen blood lymphocyte subsets

收稿日期: 2020-10-16

R383.3

基金资助:国家重点研发计划项目(No.2017YFC0909900)、青海省科技厅基础研究计划项目(No.2018-ZJ-921)联合资助

通讯作者: 曹得萍, Email: qhmccdp@163.com; ORCID: 0000-0002-1973-8879

引用本文:

曹得萍, 张耀刚, 姜博璠, 李建华, 庞明泉, 陈根. 细粒棘球蚴和多房棘球蚴感染对Balb/c小鼠淋巴细胞亚群的影响[J]. 中国人兽共患病学报, 2021, 37(5): 421-425. CAO De-ping, ZHANG Yao-gang, JIANG Bo-fan, LI Jian-hua, PANG Ming-quan, CHEN Gen. The effects of hydatid cyst and alveolar hydatid infection on lymphocyte subsets in Balb/c mice. Chinese Journal of Zoonoses, 2021, 37(5): 421-425.

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http://www.rsghb.cn/CN/10.3969/j.issn.1002-2694.2021.00.057 或 http://www.rsghb.cn/CN/Y2021/V37/I5/421

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