Bioinformatic analysis of the Hrp1 protein of Mycobacterium tuberculosis
LIU Jing1, WU Fang2, ZHANG Jie3, DONG Jiang-tao3, LIU Xiao-ling2, LIANG Su3, WANG Ju2, ZHANG Chun-jun2, WU Jiang-dong2, ZHANG Le2, ZHAO Hai-jun3, ZHANG Wan-jiang2
1. Xi'an Jiaotong University City College,Shanxi 710018,China; 2. Department of Pathophysiology, Shihezi University School of Medicine/Xinjiang High Incidence of Local and Ethnic Diseases, Key Laboratory of the Ministry of Education, Shihezi 832002, China; 3. The First Affiliated Hospital of Medical College of Shihezi University, Shihezi 832002, China
Abstract：We applied bioinformatics to predict, analyze and understand the structure, function, and biological characteristics of Hrp1 protein. Bioinformatics software, including ORF Finder, SOSUI, Protpapram, ProtScale, Signal IP, Tmpred, TMHMM, NetNGlyc, NetPhos-3.1-Servera, PSORT, WoLF PSORT, TargetP-2.0-CBS, NLStradamus, SOMPA, SWISS-MODEL, IEDB, SYFPEITHI, RANKPEP, BLAST, DNAStar and STRING, were used to predict and analyze the physicochemical properties, structure, function, biological characteristics, and epitope and protein interaction of Hrp1 protein. The results showed that Hrp1 protein has a total number of atoms of 2 166, an isoelectric point of 4.96, an instability index of 19.62, an aliphatic amino acid index of 100.35 and an average hydrophobicity of 0.042. It additionally has a transmembrane region, no signal peptide, one glycosylation site and six phosphorylation sites. Its secondary structure is dominated by α-helices, and its subcellular localization is in the cytoplasm. It is thus a stable hydrophilic protein. Bioinformatic prediction and analysis indicated that Hrp1 protein contains four B cell epitopes and multiple T cell epitopes; its interacting proteins are Rv2627c, Rv2628, Rv1738, devR, Rv2624c, TB31.7, rip3, pfkB, guaA and hspX; and it participates in a variety of biological processes. Through bioinformatic analysis, this study reveals that Hrp1 is a stable hydrophilic cytoplasmic protein that has good epitopes and may be a potential candidate factor in the diagnosis and treatment of tuberculosis. Therefore, Hrp1 is an important research target in exploring the mechanism of persistent mycobacterium tuberculosis infection and may be used as a candidate protein for the development of an anti-tuberculosis polypeptide vaccine.
2021, Vol. 37 
