Abstract:The killing effect of P-Cymene on Demodex in vitro and its mechanism of action were investigated in the present study,in which the mites were collected with cellophane tape technique,and the killing effect on Demodex was analyzed with Motic Images software with different concentrations of PCymene in vitro.The death rate of Demodex was observed and the morphological changes in D.folloculorum and D.brevis treated with P-Cymone were examined with light microscopy.It was demonstrated that P-Cymene showed powerful killing effect on Demodex in vitro and after the drug was used,the morphology and activity of Demodex altered with typical changes of excitation-spasm-chalasia-death.All the mites were killed by 100% of P-Cymene within one hour.With the decrease of P-Cymene concentration,the death rate was also dropped.The most suitable and effective concentration of P-Cymene to kill Demodex in vitro was 12.5%.In addition,the killing effect of P-Cymene on D.folliculorum was better than that on D.brevis.It is concluded that P-Cymene is a natural and highly effective drug to kill human Demodex,and the mechanism of action involves the main action of this drug on the neuromuscular system including the intense convulsive contraction and death of the affected mites.
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