The Th1/TH2 cytokine profile in peripheral mononuclear cells of patients with cystcercosis stimulated by Taenia solium antigen and its immune regulation
Abstract:To determine the level of the Th1/Th2 cytokine expression in peripheral mononuclear cells (PBMCs) of patients with cysticercosis stimulated by Taenia solium antigen and to analyze its immune regulatory effect, four-color flow cytometty was used to investigate the differentiation of T cell subsets and the Th1/Th2 cytokine profile. It was found that the percentage of CD3+/CD4+ T cell subsets ,CD4+ /CD8+ ratio and percentage of CD3+ cells secreting IFN-γ and IL-4 were higher than those of normal individuals. The percentage of CD3+ cells secreting IFN-γ in PBMCs of patients stimulated with T.solium antigen at 0, 5, 10 and 15 days were 25.42±5.53%, 30.46±4.94%, 36.52±4.73%, 38.69±5.58% respectively, and those secreting IL-4 were 2.25±0.52%, 3.00±0.57%, 3.81±0.70%, 5.03±0.73% respectively. It was also demonstrated that the percentages CD3+ cells in PBMCs of patients stimulated with onchosphere antigen presented the similar changes as well ,and the percentages of CD3+cells secreting IFN-γ/IL-4 in PBMCs of patients became gradually increased with prolongation of the antigen- induction time. These results demonstrated that in comparison with the normal individuals, abnormality in polarization level of T cells exists in PBMCs of patients with cysticercosis, who showed a significantly higher percentage of CD3+/CD4+ cells than that of normal controls, suggesting the presence of imbalance of T cell subsets and abnormality in immune responses in human cysticercosis.
〔1〕许隆祺,余森海,许淑惠.中国人体寄生虫分布与危害〔M〕.人民卫生出版社,2000,244-250.
〔2〕Cousins DJ,Lee TH,Staynov DZ.Cytokine coexpression during human Th1/Th2cell differentiation:direct evidence for coordina-ted expression of Th2cytokine〔J〕.J I mmunol,2002,169(5):2498-2506
〔3〕Grewal JS,Kaur S,Bhatti G,et al.Cellular i mmune response in human neurocysticercosis〔J〕.Parasitol Res,2000,86(6):500-503.
〔4〕Toenjes SA,Kuhn RE.The initial i mmune response during ex-peri mental cysticercosisis of the mixed Th1/Th2type〔J〕.Parasi-tol Res,2003,89(5):407-413.
〔5〕方强,孙新,夏惠,等.次氯酸钠法孵化猪带绦虫卵的实验研究〔J〕.寄生虫与医学昆虫学报,2004,11(1):19-23.
〔6〕Berger A.Th1and Th2responses:What are they?〔J〕.BMJ,2000,321(7258):424.
〔7〕Chakir H,Wang H,Lefebvre DE,et al,T-bet/GATA-3ratio as a measure of the Th1/Th2cytokine profilein mixed cell popu-lations:predominant role of GATA-3〔J〕.J I mmunol Methods,2003,278(1-2):157-169.
〔8〕Medina-Escutia E,Morales-Lopez Z,Proano JV,et al.Cellular i mmune response and Th1/Th2cytokines in human neurocystic-ercosis:lack of i mmune suppression〔J〕.J Parasitol,2001,87(3):587-590.
〔9〕Restrepo BI,Alvarez JI,Castano JA,et al.Brain granulomas in neurocysticercosis patients are associated with a Th1and Th2profile〔J〕.Infect I mmun,2001,69(7):4554-4560.
〔10〕Cortes I,Molinari JL,Solano S,et al.Taeniasoliummetacestode antigens which are protective for pigs induce Th1/Th2mixed responses in mice〔J〕.Parasitol Res,2003,90(4):273-279.