Abstract：A new molecular technique based on the use of multiplex PCR(mPCR) combined with the denaturing high-performance liquid chromatography(DHPLC) was used for the detection of Campylobacter jejuni,in which the mPCR assay was developed by using 2 sets of primers that could specifically amplify segments of the 16S rRNA and gyrA genes.The target genes fragments of the mPCR assay were 287 bp and 159 bps respectively.Analysis of 22 strains of C.jejuni indicated that this PCR system was specific and the detection limit of the mPCR in the DNA level was 10 pg/ L.This mPCR assay did not cross-react with the non-tthermo-tolerant Campylobacter and could detect 1.5 CFU/mL of Campylobacter jejuni in artificially inoculated chicken meat sample after enrichment at 42℃ in microaerophilic condition for 24 hours.By using this method 18 out of 172 chicken meat samples showed positive result in the detection of C.jejuni.These results indicated that the multiplex PCR-DHPLC assay can be used for the specific and sensitive detection of Campylobacter jejuni.
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