Abstract：In order to understand the distribution and molecular character of hantanvirus in Yunnan province,rodents were collected in residential area and mountainous area of Luxi county from September to October,2006 and rats lungs were detected by direct immunofluorescence assay. Partial S segment was amplified respectively with hantanvirus genotype-specific primers,sequenced and analyzed the homology with other known sequences. A total of 267 rodents from 4 genera and 6 species were collected from Luxi County of Yunnan Province,among which 146 from residential area (the dominant species is Rattus flavipectus) and 121 from mountain area (the dominant species is Eothenomys miletus). The positive ratio of hantavirus is 0.85%(1/117) among populated area rodents and 6.67%(6/90) among mountain area rodents,which the rodent species of positive reaction to hantavirus antigen in populated area is Rattus flavipetcus,and in mountain is Eothenomys milelusand Apodemus chevieri.LX378 was amplified by hantavirus S segment primers from 7 positive rats lungs which have been detected by immunofluorescent assay. LX378(GenBank No.HM030880)and Tula virus(AJ223601) showed higher nucleotide and amino acids homology,reached up to 81% and 89.8% respectively,while nucleotide (amino acids) homology of LX378 compared with HTN 76-118 strain and SEO L99 strain was only up to 73.9% (80.9%) and 74.4%(77.9%),respectively. Tula-like hantavirus nucleotide sequence was firstly detected from Eothenomys milelusin China. We need further study on the distribution,the natural reservoir and the pathogenicity of of Tula-like hantavirus virus in Yunnan province.
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