Abstract:To understand rat density,species constitution,positive rate of hemorrhagic fever with renal syndrome(HFRS)and molecular characteristic of Hantaviruses(HV)in Liaoning Province,a surveillance was conducted with night trapping method.The HFRS antigen was detected by IFAT and the lung cell culture was conducted with Vero-E6 cells.Then the amplified products were sequenced by RT-PCR.Results showed that there were two peak values on rat density within twelve months.The rat density was higher in village than in open country,and the sewer rats were dominant.There was no significant difference in positive rate of HFRS for four seasons or for different species of rats.The five HVs were identified into two genetic branches-four for SEO and one for HTN.The four SEO viruses were nearly in the same evolutionary branch and the only HTN virus was close to HTN76-118.In conclusion,there were two rat density summits per year in Liaoning Province and there was no relationship between the positive rate of HFRS and the species constitution or season.Liaoning province was a complex area with both SEO and HTN virus.The SEO virus was dominated and the genovariation was relatively weak.
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