Abstract:The aim of the study was to evaluate the clinical value of identification of Mycobacterium species by DNA chip,and provide scientific basis for clinical application.Clinical samples were cultured by BACTEC MGIT 960,and the species of 134 Mycobacterium isolates were identified by traditional identification methods and DNA chip,respectively.The consistent rate of Mycobacterium isolates identified by these two methods was 83.6%(112/134)and including 50 M.intracellulare,14 M.chelonae,14 M.tuberculosis,9 M.gordonae,7 M.avium,7 M.fortuitum 1 M.terrae,1 M.marinum,and 3 unidentified isolates.Of 17 Mycobacterium isolates could not be identified by DNA chips,in which 5 M.gordonae,3 M.intracellulare,2 M.chelonae,1 M.xenopi,1 M.smegmatis and 1 M.gilvum were identified by traditional identification methods.While 4 isolates could not be identified by both of these methods.It is concluded that DNA chip might be a simple,rapid,sensitive and specific method for identification of majority Mycobacterium species.