Abstract： Abstract: Objective Expressing the specific fragment of the flagellin from Borrelia brg dorf eri and purify the protein as antigen to diagnosis Lyme disease.This paper is designed to explore the diagnosis method of animal Lyme disease and Lyme disease ELISA Kit. Methods By using PCR method, to obtain the gene coding the Borrelia burgdorferi flagellin from 394bp to 798bp that exhibit low homology with related gene from other bacterial specises. Verified by DNA sequence detection, cloning and thansforming it w as expressed in E. coli BL 21 (DE3) ,then purify the recombination protein as ELISA antigen to diagnosis Lyme disease. Results The expressing of recombinant protein in host bacteria is effective and stable and the recombinant antigen could use to diagnosis Lyme disease. Conclusion:It showed the feasibility of recombinant protein as a diagnostic antigen to diagnosis Lyme disease,which lay foundations for the diagnosis of Lyme disease.
2011, Vol. 27 
