Abstract:Abstract To investigate the genotype and the characteristic of hantavirus M and S hemorrhagic fever with renal syndrome(HFRS), Lung tissues of rat were screened by indirect immunofluorescent antibody assay (IFA),and the total RNA was extracted from the serum of HFRS patients and IFA positive lung tissues. RT-PCR was applied to amplify the M and S gene of hantavirus after specific primers were designed. PCR positive product was recovered and purified before cloneing to the pMD-18 T vector and the sequencing was carried by ABI3730 gene sequencer. Compared the obtained sequencings to the HTNV and SEOV sequences of hantavirus for nucleotide homology analysis and applied MEGA5.0.4 software to construct the phylogenetic tree. The yellow-green needle-like particles were scattered in the positive lung tissues under fluorescence microscope. The PCR products of M and S gene was about 490bp and 593bp with general primers and the further amplification with HTNV typing primers to the M and S gene was respectively 242bp and 276bp, while the amplified results of M and S gene were negative with the primers of SEOV. The sequences of Hunan01,Hunan02 and Hunan03 had a high nucleotide homology to the 84FLi and SN7 strain ,which had a lower homology to the Gou3 strain. Hunan01,Hunan02 and Hunan03 were belonged to H5 subtype of HTNV. In conclusion the technology of genotyping is appropriate for hantavirus diagnosis, and the HTNV patients
and animal hosts are exist in Hunan Province.