Ultrastructure of the integument and haemocoele in [WTHX]Dermatophagoides farinae (Hughes) (Acariformes: Pyroglyphidae)
WANG Yue-ming1,2,WU Lin3,WU Ying-ying1,LI Meng1, YANG Li-tao1,HUANG Li-nian2,SUN Xin4,LIU Zhi-gang1
1.Institute of Allergy and Immunology, Medical College of Shenzhen University, Shenzhen 518060, China; 2.Respiratory Department, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China; 3.Department of Cardiology, the Third Affiliated Hospital,Sun Yat-sen University, Guangzhou 510630, China; 4.Department of Microbiology and Parasitology, Bengbu Medical College, Bengbu 233000, China
Abstract：In this study, we observed the ultrastructure of the integument and haemocoele for adult Dermatophagoides farinae with a transmission electron microscope. Twenty mites were first fixed by using 5% glutaraldehyde and postfixed by using 1% osmium tetroxide. The TEM observation was conducted after dehydration, embedding, ultrathin section, and positive staining were finished. Results showed that the curticle of D. farinae consisted of epicuticle and procuticle, in which the epicuticle is further divided into cuticulin layer, wax layer and cement layer. The procuticle is composed of endocuticle and exocuticle, which was laminated obviously and there were many pore cannals and lamellae in it. The haemocoele of Dermatophagoides farinae was surrounded by the integument, various tissues and organs are immersed in it. There were a large number of mitochondria, vacuoles, glycogen granules, and a few bacterias and phagocytes in the hemolymph. The ultrastructural observations of the integument and the haemocoele provided a more comprehensive experimental basis for the morphology research of D. farinae.
王月明,吴琳,吴莹莹,李盟,杨利桃,黄礼年,孙新,刘志刚. 粉尘螨体壁及血体腔超微结构观察[J]. 中国人兽共患病学报, 2014, 30(1): 23-26.
WANG Yue-ming,WU Lin,WU Ying-ying,LI Meng, YANG Li-tao,HUANG Li-nian,SUN Xin,LIU Zhi-gang. Ultrastructure of the integument and haemocoele in [WTHX]Dermatophagoides farinae (Hughes) (Acariformes: Pyroglyphidae). Chinese Journal of Zoonoses, 2014, 30(1): 23-26.
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