Fine-scale population genetic structure of Oncomelania hupensis based on microsatellite DNA markers
CUI Bin1,2, GUAN Wei1, YOU Ping2, LI Shi-zhu1
1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention / Key Laboratory of Parasite and Vector Biology, MOH / WHO Collaborating Center of Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China; 2. College of Life Science, Shaanxi Normal University, Xi’an 710062, China
Abstract：The genetic structure of small-scale landscape groups of Oncomelania hupensis in Songzi City, Hubei Province was identify in this study. O. hupensis snails were collected from 10 habitats in Songzi City, of which 6 polymorphic microsatellite DNA loci (T6-17, P101, D11, B14, T4-33, and C22) were carried out with GeneScan. The number of alleles (Na), heterozygosity (H), fixation index (FST) of snails in each group, genetic distance between groups, and the polymorphic information content (PIC) were calculated. Cluster analysis was then carried out based on genetic distance, and hierarchical AMOVA calculation was conducted. By certified the shells of snails, 10 groups were divided into light and ribbed shell (including shallow rib and deep rib). There were 141 alleles in total detection on 10 populations and 20-34 alleles in each locus, which were detected for 23.5 on average. The average number of alleles in 6 loci was 1.575 and the number of alleles in each locus was uneven, showing large numerical differences ranged from 0.445 to 3.060. The average observed heterozygosity (Ho) ranged from 0.438 to 0.698, being the lowest in the light shells of Tuanshan population and the highest in the deep ribs of Mamu Kou Village population; the average expected heterozygosity (He) ranged from 0.589 to 0.892, being the lowest in the shallow ribs of Desheng Village population and the highest in the deep ribs of Mamu Kou Village population. The range of FST value between paired populations was from -0.015 64 to 0.252 47, and the polymorphic information content in the population ranged from 0.528-0.857, showing a high polymorphism. Hierarchical AMOVA calculations showed that inter-individual variation of the snails occupied 88.4% of the total variations. Cluster analysis revealed that the three ribbed shell population in Munu Kou Village, Hengti Village and Yixing Village first clustered to the three light shell population in Mashizizu Village, Mingzhu Village and Tuqiao Village, then clustered to the light shell population in Tuanshan Village and Jiama Cao Village with the two shallow rib population in Desheng Village and Tianmu He Village. Under the different landscape environment of Songzi Area, there were different shells presenting on the morphology of O. hupensis. Although there was a rich diversity on O. hupensis of Songzi City, the genetic differences mostly present in individuals. Different groups didn’t show the significant genetic differentiation among the different shell morphology of O. hupensis.
崔斌, 官威, 尤平, 李石柱. 基于微卫星标记的不同壳型湖北钉螺小尺度景观群体遗传结构研究[J]. 中国人兽共患病学报, 2014, 30(7): 701-708.
CUI Bin, GUAN Wei, YOU Ping, LI Shi-zhu. Fine-scale population genetic structure of Oncomelania hupensis based on microsatellite DNA markers. Chinese Journal of Zoonoses, 2014, 30(7): 701-708.
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2014, Vol. 30 
