Effect of curcumin on superantigen TSST-1 induced inflammatory cytokines by splenocytes
WEN Xiao-ting1, HUANG Yuan-ming2, WANG Zhi-hao2, REN Zhi-hong2, LU Shan2
1.School of Public Health, Shanxi Medical University, Taiyuan 030001, China; 2.National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Abstract：In the present study, we aimed to observe the effect of curcumin on TSST-1-induced inflammatory cytokines in splenocytes of mouse and provide evidence for the further study on the effect of curcumin on inflammatory shock. Lactate dehydrogenase (LDH) release assay was used to determine cytotoxicity of different doses of TSST-1 and curcumin. Inflammatory cytokines were determined by ELISA and flow cytometry. The doses of TSST-1 and curcumin we used in the present study did not cause significant cytotoxicity. TSST-1 induced higher level of IFN-γ and IL-2 production but relatively lower level of TNF-α. The production of IL-1β, IL-6 and IL-12 was undetectable. TSST-1 induced Th1 cytokines (IFN-γ and IL-2) were not IL-12-dependent which was different from LPS-induced IFN-γ. Curcumin significantly reduced IFN-γ and TNF-α production at the concentration of 15 umol/L (P<0.05), but had no effect on IL-2 production (P>0.05). It’s suggested that curcumin could significantly inhibit the production of IFN-γ and TNF-α by splenocytes induced by TSST-1, but could not affect the proliferation of T cells.
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2014, Vol. 30 
