Differential expression and function of chitinase 3-like-1 in macrophage stimulated by Sporothrix schenckii
HUANG Li-lin1, ZHANG Jin2, GAO Wen-chao2, LI Yu-zhe1, YUAN Li-yan3, HE Tai-long1, HUANG Huai-qiu1
1.Department of Dermatology and Venereology, the Third Affiliated Hospital,Sun Yat-sen University, Guangzhou 510630, China;
2.Department of Dermatology and Venereology, Sun Yat-sen Memorial Hospital,Sun Yat-sen University, Guangzhou 510120, China;
3.Department of Dermatology, Guangdong Provincial Center for Skin Diseasesand STIs Control and Prevention, Guangzhou 510500, China
We evaluated the differential expression and function of chitinase 3-like-1 in macrophage stimulated by Sporothrix schenckii and Candida albicans fungicidal ability of macrophage after stimulation with Sporothrix schenckii and Candida albicans separately was detected. The expression of CHI3L1 gene in macrophage stimulated by Sporothrix Schenckii and Candida albicans was evaluated with real-time PCR. The function of CHI3L1 protein in macrophages against the reproduction of Sporothrix schenckii and Candida albicans was detected in vitro. Results showed that macrophages could engulf and kill Sporothrix Schenckii and Candida albicans in vitro. The expression of CHI3L1 gene in macrophage stimulated by Candida albicans was increased obviously. At the same time, CHI3L1 protein can damper the reproduction of Candida albicans. However, the expression of CHI3L1 gene was not elevated when macrophage was stimulated by Sporothrix schenckii and CHI3L1 protein played little role in reproduction of Sporothrix schenckii. The expression of CHI3L1 gene in macrophage was elevated after stimulation with Candida albicans, but was not elevated with Sporothrix Schenckii. In correspondence with differential expression, CHI3L1 in macrophages could impair the reproduction of Candida albicans but had a weak function on Sporothrix schenckii which might contribute to the pathogenesis of sporotricosis.
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